“五动”课堂理念下“PCR是获取目的基因的主要方法”教学设计

Instructional Design on “PCR as the Main Method for Obtaining Target Genes” Based on the “Five-Activities” Classroom Concept

摘要: 本文基于“五动”课堂教学模式，以沪科版高中生物“PCR是获取目的基因的主要方法”为教学内容，开展针对性的教学设计与实践。教学过程中以“人胰岛素的开发制造”真实情境为线索，提出“体外如何获取目的基因”这一个关键问题，通过复习体内DNA复制知识搭建学习阶梯，将理论分析与建模活动有机结合，同时借助多元互动提升课堂参与度，并以多维度反思活动强化学生元认知能力。实践表明，该模式能将抽象的PCR原理与过程转化为学生可感知、可操作、可深度思考的教学活动，有效促进学科核心素养的落地，实现学科育人。

Abstract: Based on the “Five Activities” teaching model, this paper carries out targeted instructional design and practice, taking “PCR as the Main Method for Obtaining Target Genes” from the Shanghai Science and Technology Edition high school biology textbook as the teaching content. With the authentic situation of “the manufacturing of human insulin” as the clues, the teaching process puts forward the key question of “how to obtain target genes in vitro”. By reviewing the knowledge of in vivo DNA replication, a learning scaffold is constructed, theoretical analysis is organically integrated with modeling activities, classroom participation is enhanced through diversified interactions, and students’ metacognitive abilities are strengthened by means of multi-dimensional reflective activities. Practice results indicate that this model can transform the abstract principles and processes of PCR into perceptible, operable and in-depth thinking teaching activities for students, effectively promote the implementation of core disciplinary competencies, and realize subject-based education.