肠道菌群与腰痛的因果关系：一项两样本 孟德尔随机化研究

Causal Relationship between Gut Microbiota and Low Back Pain: A Two-Sample Mendelian Randomization Study

摘要: 目的：通过孟德尔随机化方法，明确肠道菌群是否对腰痛的发生具有因果影响，以为腰痛的预防和管理提供新的理论依据。方法：在MiBioGen GWAS中提取肠道菌群数据，IEU Open GWAS (ukb-b-1557)中提取腰痛数据。筛选与肠道菌群相关的SNP (P < 1e−05)。使用逆方差加权法分析因果关系。通过Cochrane Q检验、MR-Egger回归、加权中值法和MR-PRESSO检验水平多效性，并进行leave-one-out敏感性分析。所有分析使用R语言及相关软件包。结果：根据IVW结果，研究发现4个肠道菌群(Prevotella9、Eubacterium rectale group、Romboutsia、Eggerthella)与腰痛之间存在负相关的潜在因果关系。结论：本研究结果表明肠道菌群与腰痛之间可能存在潜在的因果关系，为后续腰痛机制的研究提供了新的视角。与此同时，这一发现为通过靶向干预肠道菌群来预防和治疗腰痛奠定了理论基础，并为相关治疗策略的开发提供了一定的科学依据。

Abstract: Objective: To determine whether gut microbiota has a causal impact on the occurrence of low back pain (LBP) using Mendelian randomization, providing new theoretical insights for the prevention and management of LBP. Methods: Gut microbiota data were extracted from the MiBioGen GWAS, and LBP data were extracted from the IEU Open GWAS (ukb-b-1557). Single nucleotide polymorphisms (SNPs) associated with gut microbiota were selected with a threshold of P < 1e−05. Causal relationships were analyzed using inverse variance weighted (IVW) method. Horizontal pleiotropy was tested using Cochrane’s Q test, MR-Egger regression, weighted median method, and MR-PRESSO. Leave-one-out sensitivity analysis was also performed. All analyses were conducted using R software and relevant packages. Results: Based on the IVW results, four gut microbiota species (Prevotella9, Eubacterium rectale group, Romboutsia, Eggerthella) were found to have a potential causal relationship with LBP, showing a negative correlation. Conclusion: The findings of this study suggest a potential causal relationship between gut microbiota and low back pain, providing new perspectives for future research on the mechanisms of LBP. Additionally, these findings lay a theoretical foundation for targeted interventions on gut microbiota in the prevention and treatment of LBP and offer scientific evidence for the development of related therapeutic strategies.