SPP1在肺腺癌中的表达及与M2型巨噬细胞 浸润的关系
Expression of SPP1 in Lung Adenocarcinoma and Its Correlation with M2-Type Macrophage Infiltration
DOI: 10.12677/acm.2026.1652020, PDF,    科研立项经费支持
作者: 刘梦玉*, 刘百龙#：安徽医科大学第二临床医学院，安徽 合肥
关键词: 肺腺癌；SPP1；肿瘤相关巨噬细胞；预后；肿瘤微环境；Lung Adenocarcinoma； SPP1； Tumor-Associated Macrophages； Prognosis； Tumor Microenvironment
摘要: 目的：探讨分泌型磷蛋白1 (SPP1)在肺腺癌中的表达及其与M2型巨噬细胞浸润的关系。方法：基于TCGA数据库及GEPIA、TIMER等平台分析SPP1在肺腺癌中的表达、生存相关性及免疫浸润特征；收集50例肺腺癌及对应癌旁组织，采用免疫组织化学和免疫荧光检测SPP1及CD206表达，并分析其临床意义。结果：生物信息学分析显示，SPP1在肺腺癌组织中高表达，并与较差总体生存及髓系免疫细胞浸润增加相关。临床样本验证显示，SPP1在肺腺癌组织中的高表达率高于癌旁组织，并与TNM分期及疾病进展相关。SPP1高表达组无进展生存期呈缩短趋势，但差异未达统计学意义，且多因素Cox分析未提示其为独立预后因素。免疫荧光结果显示，SPP1表达与CD206+ M2型巨噬细胞密度呈正相关。结论：SPP1在肺腺癌中高表达，并与肿瘤进展及M2型巨噬细胞浸润增加相关，可能参与肺腺癌免疫微环境重塑，但其独立预后价值仍需进一步验证。
Abstract: Objective: To explore the expression of secreted phosphoprotein 1 (SPP1) in lung adenocarcinoma and its correlation with M2 macrophage infiltration. Methods: The expression level of SPP1 in lung adenocarcinoma, its correlation with survival outcomes and immune infiltration characteristics were analyzed based on the TCGA database and online platforms including GEPIA and TIMER. A total of 50 pairs of lung adenocarcinoma tissues and matched adjacent non-tumor tissues were collected. Immunohistochemistry and immunofluorescence staining were performed to detect the expression of SPP1 and CD206, and their clinical significance was further analyzed. Results: Bioinformatics analysis revealed that SPP1 was highly expressed in lung adenocarcinoma tissues, which was correlated with poor overall survival and increased infiltration of myeloid immune cells. Validation of clinical samples demonstrated that the high expression rate of SPP1 in lung adenocarcinoma tissues was significantly higher than that in adjacent non-tumor tissues, and SPP1 overexpression was associated with TNM staging and disease progression. The progression-free survival tended to be shorter in the SPP1 high-expression group, whereas the difference was not statistically significant. Multivariate Cox regression analysis indicated that SPP1 was not an independent prognostic factor. Immunofluorescence results showed that SPP1 expression was positively correlated with the density of CD206+ M2 macrophages. Conclusion: SPP1 is highly expressed in lung adenocarcinoma and is closely related to tumor progression and elevated M2 macrophage infiltration. It may participate in the remodeling of the tumor immune microenvironment in lung adenocarcinoma, while its independent prognostic value remains to be further verified.
文章引用：刘梦玉, 刘百龙. SPP1在肺腺癌中的表达及与M2型巨噬细胞 浸润的关系[J]. 临床医学进展, 2026, 16(5): 2127-2140. https://doi.org/10.12677/acm.2026.1652020

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