多功能硅纳米粒子的制备及其在肿瘤细胞中的应用研究

Preparation of Multifunctional Silicon Nanoparticles and Their Application in Tumor Cells

摘要: 采用常温水相合成法制备兼具长波长荧光发射性质及单线态氧生成能力的多功能硅纳米粒子(Ce6-SiNPs)，并针对肿瘤细胞进行荧光成像与光动力治疗。利用一系列表征手段，对荧光Ce6-SiNPs进行结构、光学性质等分析；通过CCK8法和细胞成像实验考察荧光Ce6-SiNPs的生物安全性与肿瘤细胞成像效果；并开展光动力治疗相关实验，分别检测其在体外及细胞内的单线态氧生成能力，同时探究单线态氧对肿瘤细胞的杀伤情况。基于CCK8法的细胞毒性评估显示，荧光Ce6-SiNPs具有低毒性特征，满足生物应用的基本要求；肿瘤细胞成像实验证明荧光Ce6-SiNPs在肿瘤细胞中能够达到良好的荧光成像效果，可用于生物成像领域；体外及细胞内单线态氧生成能力的检测实验表明制备的荧光Ce6-SiNPs能够有效产生单线态氧，具备进行光动力治疗的核心作用基础；而细胞杀伤活性实验进一步验证了荧光Ce6-SiNPs具备肿瘤细胞杀伤能力，为肿瘤细胞精准治疗提供了兼具成像与治疗功能的新型纳米材料。

Abstract: Multifunctional silicon nanoparticles (Ce6-SiNPs) with long-wavelength fluorescence emission and singlet oxygen generation ability were prepared by aqueous synthesis at room temperature, and fluorescence imaging and photodynamic therapy were performed for tumor cells. The structure and optical properties of Ce6-SiNPs were analyzed by a series of characterization methods. The biological safety of fluorescent Ce6-SiNPs and the imaging effect of tumor cells were investigated by CCK8 method and cell imaging experiment. Experiments related to photodynamic therapy were carried out to detect its singlet oxygen production ability in vitro and in cells, and to explore the killing effect of singlet oxygen on tumor cells. Cytotoxicity evaluation based on CCK8 method shows that fluorescent Ce6-SiNPs has low toxicity and meets the basic requirements of biological application. The experiment of tumor cell imaging proves that fluorescent Ce6-SiNPs can achieve good fluorescence imaging effect in tumor cells and can be used in the field of biological imaging. The detection experiments of singlet oxygen generation ability in vitro and in cells show that the prepared fluorescent Ce6-SiNPs can effectively generate singlet oxygen, which has the core function foundation for photodynamic therapy. The cytotoxicity experiment further verified that fluorescent Ce6-SiNPs has the ability to kill tumor cells, and provided a new nano-material with both imaging and therapeutic functions for precise treatment of tumor cells.