肝癌中piR-43772表达及对迁移的影响
Expression of piR-43772 in Hepatocellular Carcinoma and Its Effect on Migration
DOI: 10.12677/acm.2026.1652120, PDF,   
作者: 孙思军：扬州大学医学部(基础医学院·公共卫生学院)病理学教研室，江苏 扬州；盐城市大丰人民医院普外科，江苏 盐城；王成海*：扬州大学医学部(基础医学院·公共卫生学院)病理学教研室，江苏 扬州
关键词: 肝癌；piR-43772；临床病理意义；增殖；迁移；Hepatocellular Carcinoma； piR-43772； Clinicopathological Significance； Proliferation； Migration
摘要: 目的：piRNA是一种非编码RNA。本研究旨在探讨piR-43772在肝癌(HCC)中的表达、临床病理意义和功能。方法：通过piRNA表达谱分析HCC和癌旁正常组织中差异性表达的piRNA，并通过qRT-PCR验证piR-43772在68例HCC中的表达。分析piR-43772表达的临床病理意义。CCK-8实验检测piR-43772对细胞增殖的影响。细胞迁移实验通过Transwell方法检测。结果：piR-43772在68例HCC中的表达水平上调，P < 0.05；piR-43772表达与肿瘤大小和病理分化密切相关，P均 < 0.05。CCK-8实验提示piR-43772对细胞增殖活性无明显影响，P均 > 0.05。Transwell实验表明在2株HCC细胞中，过表达piR-43772后细胞的迁移数目增加，P均 < 0.05；而降低piR-43772表达后细胞的迁移数目明显减少，P均 < 0.05。结论：在HCC中piR-43772表达水平上调，是一种新的促癌基因。piR-43772促进HCC细胞的迁移。本研究可能为转移性HCC的诊治提供了临床策略。
Abstract: Objective: piRNA is a non-coding RNA. The aim of this study is to investigate the expression, clinicopathological significance and function of piR-43772 in hepatocellular carcinoma (HCC). Methods: The differentially expressed piRNAs in HCC and adjacent normal tissues were analyzed by piRNA expression profile, and the expression of piR-43772 in 68 cases of HCC was verified by qRT-PCR. The clinicopathological significance of piR-43772 expression was analyzed. CCK-8 assay was used to detect the effect of piR-43772 on cell proliferation. Transwell assay was used to detect the cell migration experiments. Results: The expression of piR-43772 was up-regulated in 68 cases of HCC (P < 0.05). The expression of piR-43772 was closely related to tumor size and pathological differentiation (both P < 0.05). The CCK-8 assay indicated that piR-43772 exerted no significant effect on cell proliferative activity, with all P > 0.05. Transwell assays showed that in two HCC cell lines, the number of migrated cells was significantly increased after piR-43772 overexpression (P < 0.05). Conversely, knockdown of piR-43772 markedly reduced cell migration (P < 0.05). Conclusions: The expression level of piR-43772 is up-regulated in HCC, which is a novel oncogene. piR-43772 promoted the migration of HCC cells. This study provides may provide a clinical strategy for the diagnosis and treatment of metastatic HCC.
文章引用：孙思军, 王成海. 肝癌中piR-43772表达及对迁移的影响[J]. 临床医学进展, 2026, 16(5): 3055-3062. https://doi.org/10.12677/acm.2026.1652120

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