皮肤鳞癌中hsa_circ_0070934的表达和作用
Expression and Role of hsa_circ_0070934 in Cutaneous Squamous Cell Carcinoma
DOI: 10.12677/acm.2026.1652182, PDF,   
作者: 顾冬玲, 王成海*：扬州大学医学部(基础医学院·公共卫生学院)，病理学教研室，江苏 扬州
关键词: 皮肤鳞状细胞癌；hsa_circ_0070934；临床病理意义；增殖；生长；Cutaneous Squamous CELL Carcinoma； hsa_circ_0070934； Clinicopathological Significance； Proliferation； Growth
摘要: 目的：探讨hsa_circ_0070934在皮肤鳞状细胞癌(CSCC)中的表达、临床病理意义及促癌功能。方法：通过circRNA表达谱筛选CSCC癌组织与癌旁正常组织差异表达circRNA，qRT-PCR验证68例CSCC组织中hsa_circ_0070934表达，分析其临床病理相关性；采用CCK-8、细胞克隆形成实验检测其对细胞增殖的调控作用。结果：hsa_circ_0070934在CSCC组织中显著高表达，其表达水平与肿瘤大小、病理分化程度密切相关；过表达hsa_circ_0070934可促进CSCC细胞增殖与克隆形成，敲低hsa_circ_0070934可表达则显著抑制细胞增殖。裸鼠荷瘤实验表明敲低hsa_circ_0070934表达可抑制皮下移植瘤的体积和重量。结论：hsa_circ_0070934在CSCC中发挥促癌作用，可能参与了CSCC的恶性进展，是值得进一步研究的潜在预后生物标志物和治疗靶点。
Abstract: Objective: Circular RNA is a type of non-coding RNA. This study aimed to investigate the expression, clinicopathological significance and function of hsa_circ_0070934 in cutaneous squamous cell carcinoma (CSCC). Methods: Differentially expressed circular RNAs in CSCC tissues and adjacent normal tissues were screened by circular RNA expression profile, and the expression of hsa_circ_0070934 in 68 CSCC tissues was verified by qRT-PCR. The clinicopathological significance of hsa_circ_0070934 expression was analyzed. Cell proliferation and colony formation were detected by CCK-8 assay and cell clone formation assay, respectively. Results: The expression level of hsa_circ_0070934 was up-regulated in 68 CSCC tissues, and its expression was closely related to tumor size and pathological differentiation. Overexpression of hsa_circ_0070934 promoted the proliferation and colony formation of CSCC cells, whereas knockdown of hsa_circ_0070934 markedly inhibited cell proliferation. Subcutaneous tumor-bearing nude mouse experiments revealed that silencing hsa_circ_0070934 expression reduced the volume and weight of subcutaneous xenograft tumors. Conclusion: hsa_circ_0070934 exerts an oncogenic role in CSCC, participates in the malignant progression of CSCC, and serves as a promising prognostic biomarker and therapeutic target for further research.
文章引用：顾冬玲, 王成海. 皮肤鳞癌中hsa_circ_0070934的表达和作用[J]. 临床医学进展, 2026, 16(5): 3586-3594. https://doi.org/10.12677/acm.2026.1652182

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