线纹海马鳗弧菌拮抗菌的筛选及鉴定
Screening and Identification of Antagonistic Bacteria against Vibrio anguillarum in Hippocampus erectus
摘要: 鳗弧菌(Vibrio anguillarum)是首个被鉴定的鱼类病原弧菌，宿主广泛，感染鱼类已超50种，前期研究显示其可引发线纹海马出现“白化–死亡”综合征。为寻求生态环保的鳗弧菌感染防控方法，本研究通过点接法和滤纸片扩散法从健康线纹海马肠道内容物中筛选出一株对鳗弧菌具有显著拮抗作用的菌株，命名Ab，综合形态学分析及16S rRNA基因系统发育树分析，将其鉴定为杀鱼假交替单胞菌(Pseudoalteromonas piscicida)；生长特性测定结果表明，该菌株在20℃~36℃范围内生长速率随温度升高而加快，最适生长盐度为30‰。本研究为后续线纹海马弧菌病的生物防治提供了候选菌株和理论基础。
Abstract: Vibrio anguillarum is the first identified pathogenic vibrio species in fish, exhibiting a broad host range and infecting over 50 fish species. Previous studies have shown that it can cause an “albinism-death” syndrome in the Hippocampus erectus. To explore an eco-friendly approach for controlling V. anguillarum infection, a strain with significant antagonistic activity against V. anguillarum was isolated from the intestinal contents of healthy Hippocampus erectus using the spot inoculation method and the disk diffusion method. The strain, designated Ab, was identified as Pseudoalteromonas piscicida based on morphological analysis and 16S rRNA gene phylogenetic tree analysis. Growth characteristic assays showed that the strain’s growth rate increased with temperature within the range of 20˚C~36˚C, and its optimal growth salinity was 30‰. This study provides a candidate strain and a theoretical basis for the biocontrol of vibriosis in lined seahorses.
文章引用：黄雨欣, 杨子涵, 石念超, 杨燕菁. 线纹海马鳗弧菌拮抗菌的筛选及鉴定[J]. 水产研究, 2026, 13(2): 166-172. https://doi.org/10.12677/ojfr.2026.132019

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