鳗弧菌菌液OD值与其活菌数相关性研究
A Study on the Correlation between the OD Value and Live Bacterial Count in Vibrio anguillarum Culture
DOI: 10.12677/ojfr.2026.132025, PDF,   
作者: 杨子涵：天津农学院水产学院，天津
关键词: 鳗弧菌；OD600；活菌数；标准曲线；Vibrio anguillarum； OD600； Viable Cell Count； Standard Curve
摘要: 分光光度法和平板计数法对细菌培养液进行测定，确定细菌培养液的OD600和活菌数在对数生长期的函数关系，根据不同的需要，有效地利用和控制细菌的浓度具有重要的意义。本研究对线纹海马肠道分离出的鳗弧菌菌株H-VA，在对数增长期稀释不同浓度的细菌培养液分别通过分光光度法和活菌计数法测定菌液的OD600值和活菌数并绘制标准曲线，为鳗弧菌生物学特性研究提供数据支撑。
Abstract: Bacterial cultures were analyzed using spectrophotometry and plate counting to determine the functional relationship between OD600 and viable cell count during the logarithmic growth phase. Effectively utilizing and controlling bacterial concentrations according to specific needs is of great significance. In this study, the Vibrio anguillarum strain H-VA, isolated from the intestine of the striped seahorse, was used. Bacterial culture solutions diluted to different concentrations during the logarithmic growth phase were analyzed using spectrophotometry and direct plate counting to determine OD600 values and viable cell counts, and standard curves were plotted to provide data support for research on the biological characteristics of Vibrio anguillarum.
文章引用：杨子涵. 鳗弧菌菌液OD值与其活菌数相关性研究[J]. 水产研究, 2026, 13(2): 224-228. https://doi.org/10.12677/ojfr.2026.132025

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