中缅树鼩解偶联蛋白-1基因部分序列的扩增与分析

Amplification and Analysis of the Partial cDNA Sequence of UCP1 Gene in Tupaia belangeri

摘要: 解偶联蛋白-1 (UCP1)是分布于线粒体内膜上的一种产热蛋白，其主要功能是参与褐色脂肪组织(BAT)的产热调节和能量代谢来维持机体的能量代谢平衡。本研究通过设计简并引物进行反转录聚合酶链式反应(RT-PCR)，从中缅树鼩(Tupaia belangeri)BAT中成功获得444 bp UCP1 cDNA核心片段。推测其氨基酸序列为148个氨基酸；经结构功能分析发现，此段氨基酸序列具有32 bp UCPs 共同具有的同源序列，具有13 bp中缅树鼩BAT UCP1的特异性序列；进行氨基酸同源性序列比较分析表明，中缅树鼩与小家鼠、褐家鼠、灰仓鼠、家兔、马、猫、牛、海象、黑猩猩和大熊猫UCP1的同源性分别为80%、81%、82%、86%、85%、86%、89%、87%、83%和88%。结果表明：UCP1编码区在哺乳动物中具有较高保守性，提示UCP1在脊椎动物中扮演着重要的生理角色。

Abstract: Uncoupling protein-1 (UCP1) is a kind of heat producing protein, which distributes on the inner membrane of mitochondria. Its main function is to maintain the body’s energy metabolic balance by participating thermogenic regulation and energy metabolism in BAT. In this study, we designed primers and used reversing transcriptase polymerase chain reaction (RT-PCR). This experiment successfully obtained 444 bp BAT UCP1 cDNA core fragment of tree shrews, and we speculated that the amino acid sequence were 148 amino acids. After structure-function analysis, we found that fragment composed of these amino acid possessed 32 bp homologous sequence of UCPs and 13 bp specific sequence of UCP1 in Tupaia belangeri. After the comparison of amino acid sequence ho-mology, the results showed that the UCP1 homology between tree shrews and other animals like Mus musculus, Rattus norvegicus, Cricetulus griseus, Oryctolagus cuniculus, Equus caballus, Felis ca-tus, Bos taurus, Odobenus rosmarus, Pongo abelii and Ailuropoda melanoleuca was respectively 80%, 81%, 82%, 86%, 85%, 86%, 89%, 87%, 83% and 88%. UCP1 coding region showed higher conser-vation in mammals, suggesting that UCP1 plays an important physiological role in vertebrates.