五味子甲素对膝骨关节炎大鼠关节软骨组织 炎症的影响

Effects of Schisandrin A on Inflammation of Articular Cartilage Tissue in Knee Osteoarthritis Rats

摘要: 目的：探究五味子甲素(Schisandrin A, Sch A)对膝骨关节炎(Knee osteoarthritis, KOA)大鼠软骨组织炎症相关指标的影响及可能作用机制。方法：将SD大鼠随机分为Control组、KOA组、Sch A组(10, 20, 40 mg/kg)、INM组，构建大鼠KOA模型，连续给药7天。末次给药后，取膝关节滑膜组织。结果：与Control组相比，KOA组大鼠血清中IL-1β、IL-18、IL-6及IL-8等促炎细胞因子水平显著升高，而随着Sch A给药剂量增加，促炎因子水平逐渐降低；在炎症相关通路检测中，KOA组TLR4、NF-κB、Myd88蛋白表达水平以及NF-κB蛋白阳性表达均显著高于Control组，且随着Sch A给药剂量增加，上述蛋白表达水平及阳性表达均逐渐降低。结论：Sch A可通过抗炎作用减轻KOA损伤，且该作用可能与TLR4/NF-κB/Myd88信号通路调节有关。

Abstract: Objective: The effects and possible mechanisms of Schisandrin A (Sch A) on the inflammatory indicators of cartilage tissue in knee osteoarthritis (KOA) rats were investigated. Methods: SD rats were randomly divided into control group, KOA group, Sch A group (10, 20, 40 mg/kg) and INM group, and the rat KOA model was constructed and administered continuously for 7 days. After the last dose, synovial tissue of the knee joint is taken. Results: Compared with the control group, the serum levels of pro-inflammatory cytokines such as IL-1β, IL-18, IL-6 and IL-8 in the KOA group increased significantly, and the levels of pro-inflammatory factors decreased gradually with the increase of Sch A dose. In the detection of inflammation-related pathways, the protein expression levels of TLR4, NF-κB, Myd88 and the positive expression of NF-κB protein in the KOA group were significantly higher than those in the control group, and the expression levels and positive expressions of the above proteins gradually decreased with the increase of Sch A dose. Conclusion: Sch A can alleviate KOA damage through anti-inflammatory effect, and may improve KOA damage by regulating TLR4/NF-κB/Myd88 signaling pathway.