PCL2在乳腺癌中的表达特征及促癌机制研究

Expression Characteristics and Oncogenic Mechanism of PCL2 in Breast Cancer

摘要: 乳腺癌(BRCA)是全球最常见的恶性肿瘤之一，对女性健康构成重大威胁。研究表明，BRCA患者体内多梳蛋白2 (PCL2)表达显著上调。然而，影响BRCA的PCL2遗传特征及其潜在机制仍不明确。因此，本研究利用TIMER和TCGA数据库探讨了PCL2在BRCA中的表达水平及其临床意义。结果显示，PCL2过表达与敲低可分别引起329个和489个差异表达基因，主要富集于细胞黏附、信号传导及肿瘤相关通路。PPI网络提示，PCL2可能通过调控CCL5、NOTCH3、LEF1、CDH1、CXCL8等基因参与细胞恶性表型相关过程。此外，乳腺BRCA细胞系的PCL2敲低实验显示其表达水平存在高低差异，表明PCL2可能是癌症进展的潜在靶点。

Abstract: Breast cancer (BRCA) is one of the most common malignant tumors worldwide and poses a major threat to women’s health. Studies have shown that the expression of Polycomb-like protein 2 (PCL2) is significantly upregulated in patients with BRCA. However, the genetic characteristics of PCL2 affecting BRCA and its underlying mechanism remain unclear. Therefore, this study investigated the expression level of PCL2 and its clinical significance in BRCA using the TIMER and TCGA databases. The results showed that PCL2 overexpression and knockdown induced 329 and 489 differentially expressed genes, respectively, which were mainly enriched in cell adhesion, signal transduction, and tumor-related pathways. The PPI network suggested that PCL2 might be involved in cell malignant phenotype-related processes by regulating genes such as CCL5, NOTCH3, LEF1, CDH1, and CXCL8. In addition, PCL2 knockdown experiments in breast BRCA cell lines revealed varying expression levels, indicating that PCL2 could serve as a potential target for cancer progression.