“参芪冬润”固体饮料对小鼠肠道微生态的影响研究

Study on the Impact of “Shenqi Dongrun” Solid Beverage on Intestinal Microecology in Mice

摘要: 目的：探索“参芪冬润”固体饮料(元气四宝茶)对小鼠肠道微生态的影响，探索其对生理功能的调节机理。方法：40只BALB/c小鼠随机地划分成了对照组、低、中、高三个剂量组，随后进行连续30天的灌胃，解剖后采集盲肠内容物进行16S rDNA测序。结果：该饮料未显著改变肠道菌群的α或β多样性。但在门水平上，芽孢杆菌门的相对丰度增加，而弯曲菌门和假单胞菌门的丰度下降。在属水平上，乳杆菌属和毛螺菌科等潜在益生菌的丰度特征性上升，而螺杆菌属等机会致病菌的丰度下降。中剂量组(1.25 g/kg∙d)的效果最为显著。结论：“参芪冬润”固体饮料的安全性较好，具备调节肠道菌群结构、增加益生菌丰度的能力，其作用机制可能与其含有的多糖成分作为益生元和促进SCFAs产生增加有关。

Abstract: Objective: To investigate the effects of “Shenqi Dongrun” solid beverage (Yuanqi Sibao Tea) on intestinal microecology of mice and explore its regulatory mechanisms on physiological functions. Methods: Forty BALB/c mice were randomly divided into a control group and low-, medium-, and high-dose groups. After 30 consecutive days of intragastric administration, cecal contents were collected for 16S rDNA sequencing. Results: The beverage did not significantly alter the α- or β-diversity of the gut microbiota. However, at the phylum level, the relative abundance of Firmicutes increased, while the abundances of Campylobacterota and Pseudomonadota decreased. At the genus level, the abundances of potential probiotics such as Lactobacillus and Lachnospiraceae characteristically increased, whereas the abundance of opportunistic pathogens such as Helicobacter decreased. The medium-dose group (1.25 g/kg∙d) exhibited the most pronounced effects. Conclusion: The “Shenqi Dongrun” solid beverage was well-tolerated and capable of modulating gut microbiota structure and increasing probiotic abundance, potentially through its polysaccharide components acting as prebiotics and promoting SCFA production.