腐殖酸及AQDS介导强化厌氧生物还原六价铬的研究
Enhanced Anaerobic Bioreduction of Hexavalent Chromium Mediated by Humic Acids and AQDS
DOI: 10.12677/wpt.2026.143016, PDF,   
作者: 赵刘柱：上海市政工程设计研究总院(集团)有限公司，上海
关键词: 腐殖酸；AQDS；厌氧生物还原；六价铬；Humic Acid； AQDS； Anaerobic Biological Reduction； Hexavalent Chromium
摘要: 六价铬(Cr6+)是工业废水中典型的有毒有害重金属离子，易溶于水，毒性大，氧化性强。厌氧生物还原Cr6+是以有机物作为电子供体，Cr6+作为电子受体进行异化呼吸，将Cr6+还原成低毒的三价铬(Cr3+)的过程；但厌氧生物还原Cr6+的方法还存在还原速率低，还原效果不好的问题。本文通过添加腐殖酸(HA)和AQDS介导强化厌氧生物还原Cr6+的效果，并考察了厌氧还原菌胞内总铬、活性氧簇(ROS)和还原力(NADH)的含量变化。结果表明：HA及AQDS对厌氧生物还原Cr6+均有明显的促进作用，且AQDS的作用强于HA；HA及AQDS可以促进电子从NADH转移给胞外的Cr6+降低还原菌胞内NADH的含量，减少了还原菌胞内总铬的积累，降低还原菌胞内ROS的含量，并且AQDS的降低效果强于HA。
Abstract: Hexavalent chromium (Cr6+) is a typical toxic and harmful heavy metal ion in industrial wastewater. It is easily soluble in water, with high toxicity and strong oxidation. Anaerobic biological reduction of Cr6+ is a process in which organic matter is used as an electron donor, Cr6+ is used as an electron acceptor for heterogeneous respiration, and Cr6+ is reduced to a low-toxic trivalent chromium (Cr3+). However, the method of reducing Cr6+ by anaerobic organisms also has the problems of low reduction rate and poor reduction effect. In this paper, the effect of anaerobic biological reduction of Cr6+ was mediated by the addition of humic acid (HA) and AQDS, and the changes of total chromium, reactive oxygen species (ROS) and reducing power (NADH) in anaerobic reducing bacteria were investigated. The results showed that HA and AQDS could promote the reduction of Cr6+ by anaerobic biological activity, and the effect of AQDS was stronger than that of HA. HA and AQDS could promote the transfer of electrons from NADH to extracellular Cr6+ to reduce the content of NADH in the reducing bacteria. It reduces the accumulation of total chromium in the cells of the reducing bacteria, reduces the content of ROS in the reducing bacteria, and the effect of AQDS is stronger than that of HA.
文章引用：赵刘柱. 腐殖酸及AQDS介导强化厌氧生物还原六价铬的研究[J]. 水污染及处理, 2026, 14(3): 145-152. https://doi.org/10.12677/wpt.2026.143016

参考文献

[1] 刘婉, 李泽琴. 水中铬污染治理的研究进展[J]. 广东微量元素科学, 2007(9): 5-9.
[2] Costa, M. (2003) Potential Hazards of Hexavalent Chromate in Our Drinking Water. Toxicology and Applied Pharmacology, 188, 1-5. [Google Scholar] [CrossRef] [PubMed]
[3] 铁合金工业污染物排放标准: GB 28666-2012 [S]. 北京: 中国环境科学出版社, 2012.
[4] 夏险, 李明顺, 武士娟, 等. 微生物铬转化和抗性机制与生物修复研究进展[J]. 微生物学通报, 2017, 44(7): 1668-1675.
[5] 黄雪娜. 异化金属还原细菌厌氧转化对硝基苯胂酸和Cr(Ⅵ)机制的研究[D]: [硕士学位论文]. 合肥: 中国科学技术大学, 2018.
[6] Van Trump, J.I., Sun, Y. and Coates, J.D. (2006) Microbial Interactions with Humic Substances. In: Advances in Applied Microbiology, Elsevier, 55-96. [Google Scholar] [CrossRef] [PubMed]
[7] Watanabe, K., Manefield, M., Lee, M. and Kouzuma, A. (2009) Electron Shuttles in Biotechnology. Current Opinion in Biotechnology, 20, 633-641. [Google Scholar] [CrossRef] [PubMed]
[8] Rosso, K.M., Smith, D.M.A., Wang, Z., Ainsworth, C.C. and Fredrickson, J.K. (2004) Self-Exchange Electron Transfer Kinetics and Reduction Potentials for Anthraquinone Disulfonate. The Journal of Physical Chemistry A, 108, 3292-3303. [Google Scholar] [CrossRef] 
[9] Fung, K.F., Zhang, Z.Q., Wong, J.W.C. and Wong, M.H. (1999) Fluoride Contents in Tea and Soil from Tea Plantations and the Release of Fluoride into Tea Liquor during Infusion. Environmental Pollution, 104, 197-205. [Google Scholar] [CrossRef] 
[10] Doong, R. and Chiang, H. (2005) Transformation of Carbon Tetrachloride by Thiol Reductants in the Presence of Quinone Compounds. Environmental Science & Technology, 39, 7460-7468. [Google Scholar] [CrossRef] [PubMed]
[11] Choi, O. and Hu, Z. (2008) Size Dependent and Reactive Oxygen Species Related Nanosilver Toxicity to Nitrifying Bacteria. Environmental Science & Technology, 42, 4583-4588. [Google Scholar] [CrossRef] [PubMed]
[12] Tian, T., Qiao, S., Yu, C. and Zhou, J. (2019) Effects of Nano-Sized MnO2 on Methanogenic Propionate and Butyrate Degradation in Anaerobic Digestion. Journal of Hazardous Materials, 364, 11-18. [Google Scholar] [CrossRef] [PubMed]
[13] Horitsu, H., Futo, S., Miyazawa, Y., Ogai, S. and Kawai, K. (1987) Enzymatic Reduction of Hexavalent Chromium by Hexavalent Chromium Tolerant Pseudomonas Ambigua G-1. Agricultural and Biological Chemistry, 51, 2417-2420. [Google Scholar] [CrossRef] 
[14] 杨雪. 强化污泥厌氧发酵产酸的效能及发酵液碳源的利用研究[D]: [博士学位论文]. 哈尔滨: 哈尔滨工业大学, 2012.
[15] Thatoi, H., Das, S., Mishra, J., Rath, B.P. and Das, N. (2014) Bacterial Chromate Reductase, a Potential Enzyme for Bioremediation of Hexavalent Chromium: A Review. Journal of Environmental Management, 146, 383-399. [Google Scholar] [CrossRef] [PubMed]
[16] García, A.C., Santos, L.A., Izquierdo, F.G., Sperandio, M.V.L., Castro, R.N. and Berbara, R.L.L. (2012) Vermicompost Humic Acids as an Ecological Pathway to Protect Rice Plant against Oxidative Stress. Ecological Engineering, 47, 203-208. [Google Scholar] [CrossRef] 

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