不同微生物菌剂对烤烟生长发育及生理特性的影响

Effects of Different Microbial Agents on Growth and Physiological Characteristics of Flue-Cured Tobacco

摘要: 为了筛选出作用于烤烟的不同微生物菌剂及施用浓度，本试验以云烟87为研究对象，设置对照组(ck)，将光合细菌稀释500 (g1)、300 (g2)、100 (g3)倍，小球藻稀释50 (x1)、100 (x2)、150 (x3)后喷施于烤烟叶片，对不同处理下烤烟叶片的生理特性进行比较。结果表明：(1) 与对照组相比，g2处理的茎围有显著提高，增幅为24.96%，g1、g2和g3处理的茎高有显著提高，增幅分别为19.85%、24.26%、18.75%，x3处理的最大叶长有显著提高，增幅为11.09%，x1、x2、x3处理的根系活力有显著提高，增幅分别为4.00%、7.78%、9.54%。(2) 与对照组相比，各处理的SOD活性均有显著性提高，其中x1处理增幅最大，为41.62%，x2处理的POD活性有显著提高，增幅为23.25%，各处理的CAT活性均有显著性提高，其中x1、x2处理增幅较大，分别为59.9%、63.09%。(3) 与对照组相比，g2、x2、x3处理的MDA含量有显著降低，降幅分别为15.51%、20.77%、23.67%。(4) 与对照组相比，各处理的可溶性蛋白和脯氨酸含量均有显著性提高。隶属函数综合分析结果表明，x3处理综合得分最高。

Abstract: In order to screen out the different microbial agents and application concentrations acting on flue-cured tobacco, Yunyan 87 was used as the research object in this experiment, and the control group (ck) was set up. Photosynthetic bacteria were diluted 500 (g1), 300 (g2), 100 (g3) times, and chlorella were diluted 50 (x1), 100 (x2), 150 (x3) and sprayed on flue-cured tobacco leaves to compare the physiological characteristics of flue-cured tobacco leaves under different treatments. The results showed that: (1) Compared with the control group, the stem circumference of g2 treatment was significantly increased by 24.96%, the stem height of g1, g2 and g3 treatments was significantly increased by 19.85%, 24.26% and 18.75% respectively, the maximum leaf length of x3 treatment was significantly increased by 11.09%, and the root activity of x1, x2 and x3 treatments was significantly increased by 4.00%, 7.78% and 9.54% respectively. (2) Compared with the control group, the SOD activity of each treatment was significantly increased, among which the x1 treatment increased the most, which was 41.62%, the POD activity of the x2 treatment was significantly increased, which was 23.25%, and the CAT activity of each treatment was significantly increased. Among them, the x1 and x2 treatments increased significantly, which were 59.9% and 63.09%, respectively. (3) Compared with the control group, the MDA content of g2, x2 and x3 treatments decreased significantly by 15.51%, 20.77% and 23.67%, respectively. (4) Compared with the control group, the soluble protein and proline content of each treatment were significantly increased. The comprehensive analysis results of membership function showed that the comprehensive score of g3 treatment was the highest.