基于网络药理学探讨钝顶螺旋藻改善高血压的作用机制研究

To Explore the Mechanism of Spirulina Platensis in Improving Hypertension Based on Network Pharmacology

摘要: 目的：采用网络药理学筛选螺旋藻活性成分，寻找其改善高血压的核心靶点，探讨作用机制。方法：通过文献及SWISS Target Prediction数据库筛选螺旋藻活性成分及作用靶点；以“Hypertension”为关键词从GeneCards、OMIM、Drugbank和TTD数据库中检索疾病靶点，取交集，构建PPI网络；通过DAVID进行GO和KEGG分析，利用Cytoscape 构建成分–靶点–通路图。结果：共获得螺旋藻活性成分相关靶点754个，203个靶点与高血压相关，SRC、CTNNB1、EGFR、STAT3、TP53、AKT1、PIK3CA、CYP3A4等核心靶点；通过GO和KEGG分析收集到836条BP、88条CC、188条MF条目，167条KEGG信号通路。结论：本研究体现了螺旋藻有效成分可改善高血压作用，为后续机制验证实验指明了方向。

Abstract: Objective: To screen the active components of spirulina by network pharmacology, to find the core targets for improving hypertension, and to explore the mechanism of action. Methods: The active components and targets of spirulina were screened by literature and SWISS Target Prediction database. The disease targets were retrieved from GeneCards, OMIM, Drugbank and TTD databases with ‘Hypertension’ as the key word, and the intersection was taken to construct the PPI network. GO and KEGG analysis were performed by DAVID, and the component-target-pathway diagram was constructed by Cytoscape. Results: 754 gene targets were obtained from the effective components of spirulina, and 203 targets were related to hypertension, such as SRC, CTNNB1, EGFR, STAT3, TP53, AKT1, PIK3CA, CYP3A4 and other core targets. Through GO and KEGG analysis, 836 BP, 88 CC, 188 MF items and 167 KEGG signaling pathways were collected. Conclusion: This study shows that the effective components of spirulina can improve hypertension, and points out the direction for subsequent mechanism verification experiments.