植物乳酸杆菌联合向日葵茎髓多糖抑制肠癌肝转移的粪便代谢组学研究

A Fecal Metabolomics Study on the Inhibition of Intestinal Cancer Liver Metastasis by Lactobacillus plantarum Combined with Sunflower Stem Pith Polysaccharides

摘要: 通过建造小鼠结直肠癌模型进行分组给药，再对小鼠粪便进行取样并且进行衍生化处理，进而对植物乳酸杆菌(Lactobacillus plantarum)联合向日葵茎髓(Helianthus annuus L.)多糖抑制结直肠癌肝转移的粪便代谢组学研究。本研究阐明多糖通过调节初级胆汁酸合成(尤其是结合阶段)，激活FXR等受体，改善脂代谢稳态；乳酸菌(LP)选择性下调脱氧肌苷以维持嘌呤代谢与基因组稳定；两者联合可抑制赖氨酸向多胺的分流，减轻炎症并优化代谢流向。上述作用共同指向初级胆汁酸、脱氧肌苷和赖氨酸等关键代谢物，通过改善肠道环境、逆转化疗耐药及增强屏障功能，发挥协同抗肠癌肝转移的效应，为代谢－肿瘤干预提供了新思路。

Abstract: By constructing a mouse model of colorectal cancer and administering drugs in groups, mouse feces were sampled and derivatized for a fecal metabolomics study on the inhibition of colorectal cancer hepatic metastasis by Lactobacillus plantarum combined with Helianthus annuus L. polysaccharides. This study elucidates that polysaccharides regulate primary bile acid synthesis (especially the conjugation stage), activate receptors such as FXR, and improve lipid metabolism homeostasis; Lactobacillus plantarum (LP) selectively downregulates deoxyinosine to maintain purine metabolism and genome stability; the combination of both inhibits the diversion of lysine to polyamines, alleviates inflammation, and optimizes metabolic flow. The above effects collectively point to key metabolites such as primary bile acids, deoxyinosine, and lysine, which exert synergistic anti-colorectal cancer hepatic metastasis effects by improving the intestinal environment, reversing chemoresistance, and enhancing barrier function, providing new insights for metabolism-tumor intervention.