高血压患者KLRB1⁺IL7R⁺γδT细胞与GZMK的关系

The Relationship between KLRB1⁺IL7R⁺γδT Cells and GZMK in Patients with Hypertension

摘要: 目的：探讨高血压患者外周血γδT细胞亚群变化及其与颗粒酶K (GZMK)水平的关系。方法：纳入前期高血压临床样本库中60例流式检测亚组研究对象，其中对照组30例、高血压组30例。采用流式细胞术检测γδT细胞比例及KLRB1⁺IL7R⁺γδT细胞比例，采用ELISA检测血清GZMK水平。结果：高血压组GZMK水平高于对照组(U = 276.0, P = 0.010)。γδT细胞总比例组间差异无统计学意义(U = 354.5, P = 0.160)，但高血压组KLRB1⁺IL7R⁺γδT细胞比例升高(U = 311.0, P = 0.041)。KLRB1⁺IL7R⁺γδT细胞比例与GZMK水平呈弱正相关(r_s = 0.304, P = 0.018)。结论：高血压患者外周血KLRB1⁺IL7R⁺γδT细胞亚群升高，并与GZMK水平呈弱正相关，提示该亚群可能与高血压状态下GZMK相关免疫异常有关。由于本研究为小样本横断面研究，上述结果仅能提示关联，尚不能推断因果关系。

Abstract: Objective: To investigate changes in peripheral blood KLRB1⁺IL7R⁺γδT-cell subsets and their relationship with granzyme K (GZMK) levels in patients with hypertension. Methods: Sixty subjects from a previous clinical sample bank were included in the flow cytometry subgroup, including 30 controls and 30 hypertensive patients. Flow cytometry was used to detect the proportions of total γδT cells and KLRB1⁺IL7R⁺γδT cells, and serum GZMK levels were measured by ELISA. Results: Serum GZMK levels were higher in hypertensive patients than in controls (U = 276.0, P = 0.010). Total γδT-cell proportions showed no significant between-group difference (U = 354.5, P = 0.160), whereas KLRB1⁺IL7R⁺γδT-cell proportions were elevated in the hypertension group (U = 311.0, P = 0.041). KLRB1⁺IL7R⁺γδT-cell proportion was weakly positively correlated with serum GZMK level (r_s = 0.304, P = 0.018). Conclusion: Elevated KLRB1⁺IL7R⁺γδT-cell proportions and their weak positive correlation with serum GZMK suggest that this subset may be associated with GZMK-related immune dysregulation in hypertension. Given the small-sample cross-sectional design, these findings indicate associations rather than causality.