石蒜碱抑制人前列腺癌细胞恶性表型的作用 研究

Study on the Role of Lycorine in Inhibiting the Malignant Phenotype of Human Prostate Cancer Cells

摘要: 目的：旨在探讨传统中草药石蒜活性成分石蒜碱(LYC)对人前列腺癌细胞DU-145的生物活性影响包括抑制肿瘤细胞增殖、迁移及体内成瘤活性。方法：实验设计采用3组方案：空白对照组、LYC 10 μmol/L处理组及LYC 100 μmol/L处理组。DU-145细胞在标准培养条件下生长，各组分别加入相应浓度的LYC进行处理。48小时后，通过显微摄影记录细胞形态，采用CCK-8法评估细胞增殖活性，运用划痕愈合实验测定细胞迁移速率。裸鼠皮下成瘤实验评估与多西他赛(DTX)联用体内抑瘤效果。结果：与空白对照相比，LYC在10 μmol/L和100 μmol/L浓度下均能显著抑制DU-145细胞增殖和克隆形成，且抑制作用呈现剂量依赖性(P < 0.05)。体内实验进一步证实，LYC增强DTX对PCa的治疗作用，有效降低肿瘤在动物模型中的生长能力。结论：LYC与DTX协同干预能有效抑制PCa细胞增殖、迁移以及体内成瘤等恶性生物学行为，提示其在PCa治疗领域具有潜在的应用前景。

Abstract: Objective: The aim of this study is to explore the biological activity of the active component of traditional Chinese medicine, lycorine (LYC), on human prostate cancer cells DU-145, including its effects on inhibiting tumor cell proliferation, migration, and in vivo tumor formation. Methods: The experimental design included three groups: blank control group, LYC 10 μmol/L treatment group, and LYC 100 μmol/L treatment group. DU-145 cells were grown under standard culture conditions, and each group was treated with the corresponding concentration of LYC. After 48 hours, cell morphology was recorded using microscopic photography, cell proliferation activity was evaluated by CCK-8 method, and cell migration rate was determined using scratch wound healing assay. The in vivo tumor formation experiment in nude mice was used to evaluate the in vivo anti-tumor effect in combination with docetaxel (DTX). Results: Compared with the blank control group, LYC at concentrations of 10 μmol/L and 100 μmol/L could significantly inhibit the proliferation and colony formation of DU-145 cells, and the inhibitory effect was dose-dependent (P < 0.05). The in vivo experiments further confirmed that LYC enhanced the therapeutic effect of DTX on prostate cancer, effectively reducing the growth ability of tumors in the animal model. Conclusion: The synergistic intervention of LYC and DTX can effectively inhibit the malignant biological behaviors of prostate cancer cells, such as proliferation, migration, and in vivo tumor formation, suggesting its potential application in the treatment of prostate cancer.