浒苔硫酸化鼠李聚糖缓解葡聚糖硫酸钠诱导的 溃疡性结肠炎作用研究
Study on the Alleviative Effect of the Sulfated Rhamnan from Enteromorpha prolifera on Dextran Sulfate Sodium-Induced Ulcerative Colitis
摘要: 为探究浒苔多糖对溃疡性结肠炎的影响。本研究采用冷水提法从浒苔中提取多糖，经分离纯化得到浒苔多糖(EP)，其分子量为132.6 kDa，总糖含量为64.6%，硫酸基含量为15.1%。单糖组成分析发现EP主要由鼠李糖组成，其次还含有葡萄糖和阿拉伯糖。在葡聚糖硫酸钠(DSS)诱导的溃疡性结肠炎小鼠模型中，EP干预可显著改善小鼠体重、结肠长度及DAI评分，修复结肠组织病理损伤，减少炎性浸润并保护杯状细胞。EP能够上调紧密连接蛋白ZO-1和occludin的表达，增强肠道屏障功能；还能通过抑制NLRP3激活，下调促炎因子TNF-α和IL-1β水平，并促进抗炎因子IL-10的分泌，从而缓解肠道炎症反应。同时，EP可有效增加肠道菌群的多样性，降低厚壁菌门/拟杆菌门比例，促进Faecalibaculum和Lactococcus等有益菌增殖，抑制Escherichia-Shigella和Staphylococcus等致病菌生长，从而重塑肠道微生态。综上，浒苔多糖EP可通过修复肠道屏障、抑制炎症反应、调节肠道菌群等，有效缓解DSS引起的结肠炎症。研究结果为浒苔多糖改善溃疡性结肠炎提供实验数据，为浒苔资源高值化利用提供理论依据。
Abstract: To investigate the effect of Enteromorpha prolifera polysaccharide on ulcerative colitis. In this study, E. prolifera polysaccharide (EP) was extracted and purified from E. prolifera by cold water extraction. EP had a molecular weight of 132.6 kDa, a total sugar content of 64.6%, and a sulfate group content of 15.1%. Monosaccharide composition analysis revealed that EP was mainly composed of rhamnose, followed by glucose and arabinose. In a mouse model of ulcerative colitis induced by dextran sulfate sodium (DSS), EP intervention significantly improved body weight, colon length, and DAI score, repaired colonic histopathological damage, reduced inflammatory infiltration, and protected goblet cells. EP upregulated the expression of tight junction proteins ZO-1 and occludin to enhance intestinal barrier function. It also alleviated intestinal inflammation by inhibiting NLRP3 activation, downregulating the levels of pro-inflammatory cytokines TNF-α and IL-1β, and promoting the secretion of anti-inflammatory cytokine IL-10. Meanwhile, EP effectively increased the diversity of gut microbiota, decreased the Firmicutes/Bacteroidetes ratio, promoted the proliferation of beneficial bacteria such as Faecalibaculum and Lactococcus, and inhibited the growth of pathogenic bacteria including Escherichia-Shigella and Staphylococcus, thereby remodeling intestinal microecology. In conclusion, E. prolifera polysaccharide EP can effectively alleviate DSS-induced colitis by repairing the intestinal barrier, suppressing inflammatory responses, and regulating gut microbiota. These findings provide experimental data for the application of E. prolifera polysaccharide in ameliorating ulcerative colitis and a theoretical basis for the high-value utilization of E. prolifera resources.
文章引用：杨德昭, 孙海红, 刘钟毓, 李惠汝, 张楚乔, 程昭菁, 李红燕. 浒苔硫酸化鼠李聚糖缓解葡聚糖硫酸钠诱导的 溃疡性结肠炎作用研究[J]. 生物医学, 2026, 16(4): 735-748. https://doi.org/10.12677/hjbm.2026.164075

参考文献

[1] 弓艳霞, 唐艳萍, 牛薇, 刘思邈, 徐阳. 溃疡性结肠炎发病机制及治疗研究进展[J]. 中国中西医结合外科杂志, 2018, 24(4): 512-516.
[2] 林轩永. 溃疡性结肠炎治疗现状及进展[J]. 临床医药文献电子杂志, 2020, 7(12): 191-193.
[3] Chen, X., Li, X., Sun-Waterhouse, D., Zhu, B., You, L. and Hileuskaya, K. (2021) Polysaccharides from Sargassum fusiforme after Uv/H2O2 Degradation Effectively Ameliorate Dextran Sulfate Sodium-Induced Colitis. Food & Function, 12, 11747-11759. [Google Scholar] [CrossRef] [PubMed]
[4] Liu, X., Zhang, Y., Li, W., Zhang, B., Yin, J., Liuqi, S., et al. (2022) Fucoidan Ameliorated Dextran Sulfate Sodium-Induced Ulcerative Colitis by Modulating Gut Microbiota and Bile Acid Metabolism. Journal of Agricultural and Food Chemistry, 70, 14864-14876. [Google Scholar] [CrossRef] [PubMed]
[5] Xue, M., Ji, X., Liang, H., Liu, Y., Wang, B., Sun, L., et al. (2018) The Effect of Fucoidan on Intestinal Flora and Intestinal Barrier Function in Rats with Breast Cancer. Food & Function, 9, 1214-1223. [Google Scholar] [CrossRef] [PubMed]
[6] Wassie, T., Niu, K., Xie, C., Wang, H. and Xin, W. (2021) Extraction Techniques, Biological Activities and Health Benefits of Marine Algae Enteromorpha prolifera Polysaccharide. Frontiers in Nutrition, 8, Article 747928. [Google Scholar] [CrossRef] [PubMed]
[7] Zhang, S., Zhang, M., Li, W., Ma, L., Liu, X., Ding, Q., et al. (2023) Research Progress of Natural Plant Polysaccharides Inhibiting Inflammatory Signaling Pathways and Regulating Intestinal Flora and Metabolism to Protect Inflammatory Bowel Disease. International Journal of Biological Macromolecules, 253, Article ID: 126799. [Google Scholar] [CrossRef] [PubMed]
[8] Roy, D., Sobuj, M.K.A., Islam, M.S., Haque, M.M., Islam, M.A., Islam, M.M., et al. (2024) Compositional, Structural, and Functional Characterization of Fucoidan Extracted from Sargassum polycystum Collected from Saint Martin’s Island, Bangladesh. Algal Research, 80, 103542. [Google Scholar] [CrossRef] 
[9] Li, H., Xie, W., Qiao, X., Cui, H., Yang, X. and Xue, C. (2020) Structural Characterization of Arabinogalactan Extracted from Ixeris chinensis (Thunb.) Nakai and Its Immunomodulatory Effect on RAW264.7 Macrophages. International Journal of Biological Macromolecules, 143, 977-983. [Google Scholar] [CrossRef] [PubMed]
[10] Yu, M., Xu, G., Qin, M., Li, Y., Guo, Y. and Ma, Q. (2022) Multiple Fingerprints and Spectrum-Effect Relationship of Polysaccharides from Saposhnikoviae radix. Molecules, 27, Article 5278. [Google Scholar] [CrossRef] [PubMed]
[11] 钱轩韬, 郝微微, 张尔馨, 时艺榕, 刘畅, 尚小青. 基于NLRP3探究健脾化湿颗粒对溃疡性结肠炎小鼠血管生成与黏附分子表达的影响[J/OL].中国实验动物学报: 1-11.
https://link.cnki.net/urlid/11.2986.q.20260411.1131.012, 2026-06-22.
[12] Ma, X., Wang, D., Liu, Y., Liu, B., Feng, X. and Yang, W. (2023) Transcriptomics and Experimental Validation-Based Approach to Understand the Effect and Mechanism of Huangqin Tang Interfeience with Colitis Associated Colorectal Cancer. Heliyon, 9, e13739. [Google Scholar] [CrossRef] [PubMed]
[13] Peuhkuri, K. (2010) Even Low-Grade Inflammation Impacts on Small Intestinal Function. World Journal of Gastroenterology, 16, 1057. [Google Scholar] [CrossRef] [PubMed]
[14] Son, S., Suh, H.J. and Shin, K. (2024) Characterization of a Novel Sulfated-Rhamnoglucuronan Isolated from Korean Seaweed Ulva pertusa and Its Efficacy for Treatment of Inflammatory Bowel Disease in Mice. Carbohydrate Polymers, 342, 122373. [Google Scholar] [CrossRef] [PubMed]
[15] 蔡聪会, 罗晓英, 张炳勇. 肠黏膜杯状细胞功能缺陷在溃疡性结肠炎发病中的作用[J]. 胃肠病学和肝病学杂志, 2026, 35(1): 122-126.
[16] Li, S., Qian, Q., Yang, H., Wu, Z., Xie, Y., Yin, Y., et al. (2024) Fucoidan Alleviated Dextran Sulfate Sodium-Induced Ulcerative Colitis with Improved Intestinal Barrier, Reshaped Gut Microbiota Composition, and Promoted Autophagy in Male C57BL/6 Mice. Nutrition Research, 122, 1-18. [Google Scholar] [CrossRef] [PubMed]
[17] Wang, L., Zhang, P., Li, C., Xu, F. and Chen, J. (2022) A Polysaccharide from Rosa roxburghii Tratt Fruit Attenuates High-Fat Diet-Induced Intestinal Barrier Dysfunction and Inflammation in Mice by Modulating the Gut Microbiota. Food & Function, 13, 530-547. [Google Scholar] [CrossRef] [PubMed]
[18] Fang, D. and Zhu, J. (2019) Molecular Switches for Regulating the Differentiation of Inflammatory and IL-10-Producing Anti-Inflammatory T-Helper Cells. Cellular and Molecular Life Sciences, 77, 289-303. [Google Scholar] [CrossRef] [PubMed]
[19] Qiu, P., Ishimoto, T., Fu, L., Zhang, J., Zhang, Z. and Liu, Y. (2022) The Gut Microbiota in Inflammatory Bowel Disease. Frontiers in Cellular and Infection Microbiology, 12, Article 733992. [Google Scholar] [CrossRef] [PubMed]
[20] Wu, Y., Zhang, X., Liu, X., Zhao, Z., Tao, S., Xu, Q., et al. (2024) Galactooligosaccharides and Limosilactobacillus reuteri Synergistically Alleviate Gut Inflammation and Barrier Dysfunction by Enriching Bacteroides Acidifaciens for Pentadecanoic Acid Biosynthesis. Nature Communications, 15, Article No. 9291. [Google Scholar] [CrossRef] [PubMed]
[21] Shin, N., Whon, T.W. and Bae, J. (2015) Proteobacteria: Microbial Signature of Dysbiosis in Gut Microbiota. Trends in Biotechnology, 33, 496-503. [Google Scholar] [CrossRef] [PubMed]
[22] Haque, M., Kaminsky, L., Abdulqadir, R., Engers, J., Kovtunov, E., Rawat, M., et al. (2024) Lactobacillus acidophilus Inhibits the TNF-α-Induced Increase in Intestinal Epithelial Tight Junction Permeability via a TLR-2 and PI3K-Dependent Inhibition of NF-κB Activation. Frontiers in Immunology, 15, Article 1348010. [Google Scholar] [CrossRef] [PubMed]
[23] Zhang, Y., Tu, S., Ji, X., Wu, J., Meng, J., Gao, J., et al. (2024) Dubosiella newyorkensis Modulates Immune Tolerance in Colitis via the L-Lysine-Activated AhR-IDO1-Kyn Pathway. Nature Communications, 15, Article No. 1333. [Google Scholar] [CrossRef] [PubMed]
[24] Castaño-Rodríguez, N., Underwood, A.P., Merif, J., Riordan, S.M., Rawlinson, W.D., Mitchell, H.M., et al. (2018) Gut Microbiome Analysis Identifies Potential Etiological Factors in Acute Gastroenteritis. Infection and Immunity, 86, e00060-18. [Google Scholar] [CrossRef] [PubMed]
[25] 胡付豪, 刘玉晖, 梁新丽, 杨辉, 许汉林, 韩飞. 基于肠道菌群和炎症因子探讨牛至油对溃疡性结肠炎小鼠的作用[J]. 中成药, 2023, 45(4): 1294-1300.
[26] 韩舒晨, 李柏阳, 董芷嘉, 石杰, 张正海, 李国巍, 姬妍茹, 杨庆丽, 潘静, 刘淑霞, 高宇, 肖湘, 胡明. 黑海棠果粗多糖对小鼠结肠炎的缓解作用[J/OL]. 食品工业科技: 1-20. https://link.cnki.net/doi/10.13386/j.issn1002-0306.2025100111, 2026-06-22.[CrossRef] 
[27] Yu, S., Xie, J., Guo, Q., Yan, X., Wang, Y., Leng, T., et al. (2024) Clostridium butyricum Isolated from Giant Panda Can Attenuate Dextran Sodium Sulfate-Induced Colitis in Mice. Frontiers in Microbiology, 15, Article 1361945. [Google Scholar] [CrossRef] [PubMed]
[28] Kim, W., Min, S., Kwon, H., Park, S., Jo, M.J. and Ko, G. (2023) Lactobacillus rhamnosus KBL2290 Ameliorates Gut Inflammation in a Mouse Model of Dextran Sulfate Sodium-Induced Colitis. Journal of Microbiology, 61, 673-682. [Google Scholar] [CrossRef] [PubMed]
[29] Wang, Y., Song, X., Wang, Z., Li, Z. and Geng, Y. (2023) Effects of Pine Pollen Polysaccharides and Sulfated Polysaccharides on Ulcerative Colitis and Gut Flora in Mice. Polymers, 15, Article 1414. [Google Scholar] [CrossRef] [PubMed]
[30] Ma, M., Quan, M., Zhang, J., Zhang, A., Gao, P., Shang, Q., et al. (2023) In Vitro Fermentation of Polysaccharide from Edible Alga Enteromorpha clathrata by the Gut Microbiota of Patients with Ulcerative Colitis. Nutrients, 15, Article 4122. [Google Scholar] [CrossRef] [PubMed]

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