UPLC-MS/MS测定油蒿叶片中4种内源植物激素

Determination of Four Endogenous Phytohormones in Artemisia ordosica Leaves by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry

摘要: 本文以菊科植物油蒿(Artemisia ordosica)为研究材料，采用超高效液相色谱串联质谱法对叶片中的植物激素进行分析，以探讨植物组织中的植物激素鉴定方法。油蒿叶片经异丙醇–水–盐酸(2:1:0.002, V/V/V)浸提，二氯甲烷萃取，反相C18柱分离，流动相为0.1%的乙酸水溶液和乙腈，梯度洗脱。多反应监测离子模式进行定性和定量分析，外标法定量。用该方法测定吲哚-3-乙酸、脱落酸、赤霉素和茉莉酸4种内源植物激素的回收率高于94.0%，精密度和重现性均较好(相对标准偏差为0.92%~1.56%)。对实际植物样品的分析也取得了较理想的结果，表明该方法完全适用于植物样品中植物激素含量的检测。

Abstract: Four endogenous phytohormones, indole-3-acetic acid, abscisic acid, gibberellins and jasmonic acid in the leaves of Artemisia ordosica were examined by ultra performance liquid chromatogra-phy-tandem mass spectrometry (UPLC-MS/MS) to provide effective and reliable methods for iden-tification of phytohormones in plants. The four analytes were extracted from A. ordosica leaves by isopropanol-water-hydrochloric acid (2:1:0.002, V/V/V), and partitioned by dichloromethane, and then separated by Agilent C18 column with gradient elution using acetonitrile and 0.1% acetic acid in water as mobile phases. Negative electrospray ionization with multiple reaction monitor mode was used in the detection. External standard method was used to quantify the analytes concentra-tions. The recoveries of this method were higher than 94.0%, and the relative standard deviations were 0.92% - 1.56%. The results showed that phytohormones was well separated, and the ideal quantitative data of phytohormones in A. ordosica leaves were obtained, and this method suited the purpose of the determination for phytohormones in plant material.