基于网络药理学探讨中药半枝莲治疗子宫颈癌的作用机制

Exploring the Mechanism of Action of the Chinese Herbal Medicine Scutellaria barbata in Treating Cervical Cancer Based on Network Pharmacology

摘要: 目的：基于网络药理学方法，深度探讨中药半枝莲治疗子宫颈癌的潜在多靶点作用机制。方法：通过中药系统药理学数据库(TCMSP)检索半枝莲的相关作用靶点，并从GeneCards、OMIM等疾病数据库获取子宫颈癌的致病靶点。利用STRING数据库构建PPI网络和药物–疾病–成分–共同靶点网络图，通过R语言进行GO功能和KEGG通路富集分析。结果：共获得半枝莲与子宫颈癌的共同作用靶点126个；PPI网络分析显示，TP53、JUN和AKT1等基因处于网络核心节点；GO富集分析表明，靶点主要涉及对外源刺激的反应(Response to Xenobiotic Stimulus)、膜筏(Membrane Raft)、DNA结合转录因子结合(DNA-Binding Transcription Factor Binding)等生物学过程。KEGG通路分析显示，靶点显著富集于脂质与动脉粥样硬化(Lipid and Atherosclerosis)、人巨细胞病毒感染(Human Cytomegalovirus Infection)、PI3K/AKT信号通路等相关通路。通过筛选共获得29味半枝莲的有效成分，其中最主要的两味中药成分为槲皮素(Quercetin)和木犀草素(Luteolin)。结论：研究通过网络药理学方法揭示了半枝莲可能通过多靶点、多通路协同作用治疗子宫颈癌。这些发现为半枝莲的临床应用提供了新的理论依据和治疗靶点。

Abstract: Objective: To deeply explore the potential multi-target mechanism of action of the Chinese herbal medicine Scutellaria barbata in the treatment of cervical cancer based on network pharmacology. Methods: In this study, the relevant targets of Scutellaria barbata were obtained from the TCMSP database, while disease-related targets of Cervical cancer were retrieved from disease databases such as GeneCards and OMIM. The STRING database was used to construct a protein-protein interaction (PPI) network and a drug-disease-component-common target network. Gene Ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed using the R language. Results: A total of 126 common targets between Scutellaria barbata and cervical cancer were identified. PPI network analysis indicated that genes such as TP53, JUN, and AKT1 were core nodes in the network. GO enrichment analysis showed that the targets were primarily involved in biological processes such as response to xenobiotic stimulus, membrane raft, and DNA-binding transcription factor binding. KEGG pathway analysis revealed that the targets were significantly enriched in pathways including lipid and atherosclerosis, human cytomegalovirus infection, and the PI3K/AKT signaling pathway. Through screening, 29 active components of Scutellaria barbata were identified, with quercetin and luteolin being the two main components. Conclusion: This study reveals, through network pharmacology, that Scutellaria barbata may exert its therapeutic effects in cervical cancer through the synergistic action of multiple targets and pathways. These findings provide a new theoretical basis for the clinical application of Scutellaria barbata and offer novel therapeutic targets.