基于数据挖掘探讨针灸治疗颤证核心穴位的作用机制

Exploring the Mechanism of Core Acupoints in Acupuncture Treatment for Tremor Syndrome Based on Data Mining

摘要: 目的：采用数据挖掘方法分析针灸治疗颤证的组穴规律及核心穴方的分子机制，为临床选穴与机制研究提供参考。方法：检索PubMed、CNKI等数据库，建立针灸处方数据库，利用SPSS Modeler进行关联规则分析获取核心穴方；运用Cytoscape、String数据库及Metascape工具，构建穴位–靶点–疾病网络、蛋白质互作网络，并进行GO与KEGG富集分析。结果：纳入227篇文献，核心穴方为百会、风池、四神聪、太冲、合谷、曲池、阳陵泉、三阴交、足三里。共获得潜在治疗靶点649个，与颤证交集靶点110个；经PPI拓扑分析提取核心靶点(如AKT1、BDNF、TNF、TP53、IL1B、SNCA等)。富集分析表明，核心穴方主要作用于神经退行性疾病、帕金森病、神经活性配体–受体相互作用等通路，涉及突触信号传递、膜电位调节、神经元凋亡及氧化应激等生物过程。结论：针灸治疗颤证的核心穴方通过多靶点、多通路协同发挥治疗作用，为临床选穴及机制研究提供了理论基础。

Abstract: Objective: To analyze the acupoint combination rules of acupuncture for tremor syndrome (chanzheng) using data mining methods and to explore the molecular mechanisms of the core acupoint formula, providing a reference for clinical acupoint selection and mechanistic research. Methods: Relevant literature was retrieved from databases including PubMed and CNKI to establish an acupuncture prescription database. Association rule analysis was performed using SPSS Modeler to identify the core acupoint formula. Cytoscape, the String database, and Metascape were used to construct an acupoint-target-disease network and a protein-protein interaction network, followed by GO and KEGG enrichment analyses. Results: A total of 227 articles were included. The core acupoint formula consisted of Baihui (GV20), Fengchi (GB20), Sishencong (EX-HN1), Taichong (LR3), Hegu (LI4), Quchi (LI11), Yanglingquan (GB34), Sanyinjiao (SP6), and Zusanli (ST36). A total of 649 potential therapeutic targets were identified, among which 110 intersected with tremor syndrome. Core targets (e.g., AKT1, BDNF, TNF, TP53, IL1B, SNCA) were extracted through topological analysis of the PPI network. Enrichment analysis showed that the core acupoint formula mainly acts on pathways such as neurodegeneration, Parkinson’s disease, and neuroactive ligand-receptor interaction, involving biological processes including synaptic signaling, membrane potential regulation, neuronal apoptosis, and oxidative stress. Conclusion: The core acupoint formula of acupuncture for tremor syndrome exerts therapeutic effects through multi-target and multi-pathway synergistic actions, providing a theoretical basis for clinical acupoint selection and mechanistic research.