敲低CARMA3对非小细胞肺癌中增殖、迁移以及侵袭能力的影响
The Effects of CARMA3 Downregulation on Proliferation Migration, and Invasion in Non-Small Cell Lung Cancer
DOI: 10.12677/acm.2026.1682824, PDF,    科研立项经费支持
作者: 张苗苗, 陈 亮*：四川省什邡市人民医院检验科，四川 德阳；舒增怡：四川省什邡市人民医院病理科，四川 德阳
关键词: 凋亡酶招募域和膜相关鸟苷酸激酶样蛋白3；非小细胞肺癌；NF-κB信号通路；细胞增殖；细胞凋亡；CARMA3； NSCLC； NF-κB Signaling Pathway； Cell Proliferation； Cell Apoptosis
摘要: 目的：探讨凋亡酶招募域和膜相关鸟苷酸激酶样蛋白3(CARMA3)在非小细胞肺癌(NSCLC)组织中的表达及临床意义，以及敲低CARMA3对NSCLC细胞增殖、凋亡、迁移和侵袭能力的影响以及对NF-κB信号通路(总NF-κB p65及磷酸化NF-κB p65)的调控作用。方法：采用免疫组化检测NSCLC(10例：5例肺腺癌和5例肺鳞癌)及癌旁组织中CARMA3的表达水平，分析其与临床病理特征的关系；以肺鳞癌细胞H1975和肺腺癌细胞H520为研究对象，分别敲低和过表达改变CARMA3表达水平，利用细胞增殖、划痕、侵袭及细胞凋亡等实验检测其生物学功能，Western blot检测NF-κB信号通路核心蛋白磷酸化p65 (p-p65)的表达变化。结论：CARMA3在NSCLC组织中呈高表达，敲低CARMA3可抑制NSCLC细胞增殖、迁移和侵袭，并促进细胞凋亡，其作用机制与抑制NF-κB信号通路活化有关，可能成为NSCLC治疗的潜在分子靶点。
Abstract: Objective: To investigate the expression and clinical significance of the apoptosis enzyme recruitment domain and membrane-associated guanylate kinase-like protein 3 (CARMA3) in non-small cell lung cancer (NSCLC) tissues, as well as the effects of CARMA3 knockdown on NSCLC cell proliferation, apoptosis, migration, and invasion capabilities, as well as its regulatory role in the NF-κB signaling pathway (total NF-κB p65 and phosphorylated NF-κB p65). Methods: Immunohistochemistry was used to detect CARMA3 expression levels in NSCLC (10 cases: 5 cases of lung adenocarcinoma and 5 cases of lung squamous cell carcinoma) and peritumoral tissues, and to analyze its relationship with clinical and pathological characteristics; using the lung squamous cell carcinoma cell line H1975 and the lung adenocarcinoma cell line H520 as study models, we knocked down and overexpressed CARMA3, respectively, to alter its expression levels. We assessed its biological functions using assays for cell proliferation, the scratch assay, invasion, and apoptosis, and used Western blot analysis to detect changes in the expression of phosphorylated p65 (p-p65), a core protein of the NF-κB signaling pathway. Conclusion: CARMA3 is highly expressed in NSCLC tissues. Knocking down CARMA3 inhibits NSCLC cell proliferation, migration, and invasion, and promotes apoptosis. Its mechanism of action is associated with the inhibition of NF-κB signaling pathway activation, and it may serve as a potential molecular target for NSCLC treatment.
文章引用：张苗苗, 陈亮, 舒增怡. 敲低CARMA3对非小细胞肺癌中增殖、迁移以及侵袭能力的影响[J]. 临床医学进展, 2026, 16(8): 536-547. https://doi.org/10.12677/acm.2026.1682824

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