GRF-GIF嵌合蛋白系统研究进展及木本植物应用前景
GRF-GIF Chimera System and Its Application Potential in Genetic Transformation of Woody Plants
DOI: 10.12677/br.2026.155036, PDF,    科研立项经费支持
作者: 袁千允, 李 培*：华南农业大学林学与风景园林学院，广东 广州
关键词: GRF-GIF嵌合蛋白；木本植物；遗传转化；再生效率；应用潜力；GRF-GIF Combination； Woody Plants； Genetic Transformation； Regeneration Efficiency； Application Potential
摘要: 遗传转化是基因功能解析和分子育种的关键技术，但传统组织培养依赖型转化体系在木本植物中面临再生困难、基因型依赖性强和转化效率低等核心瓶颈。生长调控因子(GRF)与GRF互作因子(GIF)形成的转录激活复合物在植物生长发育和再生能力调控中发挥核心作用。近年来，GRF-GIF嵌合蛋白系统作为一种普适性的形态发生调节策略，通过过表达嵌合体显著提升多种植物的再生与转化效率，已成功应用于杨树、大豆、小麦、玉米等作物的遗传转化和基因编辑。本文系统综述了GRF和GIF基因的结构特征与功能机制、GRF-GIF嵌合蛋白系统提升转化效率的分子基础及其在植物遗传转化中的应用进展，重点探讨该技术在木本植物中的研究现状与潜力，并对该技术在珍稀木本植物遗传改良中的未来应用前景进行了展望。
Abstract: Genetic transformation is a key technology for gene functional analysis and molecular breeding. However, traditional tissue culture-dependent transformation systems in woody plants face core bottlenecks, including difficulties in regeneration, strong genotype dependence, and low transformation efficiency. The transcriptional activation complex formed by GROWTH-REGULATING FACTOR (GRF) and GRF-INTERACTING FACTOR (GIF) plays a central role in regulating plant growth, development, and regeneration capacity. In recent years, the GRF-GIF chimera system, as a universal morphogenic regulator strategy, has significantly improved the regeneration and transformation efficiency of multiple plant species through overexpression of chimeric proteins, and has demonstrated important application value in genetic transformation and gene editing of crops such as poplar, soybean, wheat, and maize. This review systematically summarizes the structural characteristics and functional mechanisms of GRF and GIF genes, the molecular basis by which the GRF-GIF chimera system enhances transformation efficiency, and its current application status in plant genetic transformation. Emphasis is placed on analyzing the preliminary exploration results and application potential of this system in woody plants, along with prospects for future research directions, aiming to provide a theoretical reference for breakthroughs in genetic transformation technology of woody plants.
文章引用：袁千允, 李培. GRF-GIF嵌合蛋白系统研究进展及木本植物应用前景[J]. 植物学研究, 2026, 15(5): 311-317. https://doi.org/10.12677/br.2026.155036

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