通过两样本孟德尔随机化方法研究茶摄入量与全身骨密度之间的因果关系

Study on the Causal Relationship between Tea Intake and Systemic Bone Density Using a Two-Sample Mendelian Randomization Method

摘要: 背景：骨密度降低所带来的一系列症状严重影响人类生活质量，故寻找茶摄入与骨密度的因果关系对健康有重要意义。目的：利用两样本孟德尔随机化方法探究茶摄入量(Tea intake)与全身骨密度(Total body bone mineral density)之间是否存在因果关系。方法：从公开的GWAS数据库网站中获得有关的茶摄入量全基因组关联分析数据和全身骨密度全基因组关联分析数据，通过加权中位数法(Weighted median)逆方差加权法(Inverse variance weighted)、MR-Egger分析等两样本孟德尔随机化方法来评估茶摄入量与全身骨密度之间的因果关系，最后应用敏感性分析评判结论是否可靠。结果：IVW逆方差加权法(OR = 1.204308, 95% CI: 1.0620635~1.365603, P = 0.003744053)、MR-Egger分析(OR = 1.062008, 95% CI: 0.7962864~1.416400, P = 0.684541424)、加权中位数法(OR = 1.178897, 95% CI: 0.9937853~1.398488, P = 0.058963289)；异质性检验(IVW Q-pval = 0.11, MR-Egger Q-pval = 0.12)、多效性检验(pval = 0.3474897)。结论：孟德尔随机化结果显示茶摄入量与全身骨密度风险之间存在因果关系，且饮茶有助于提高全身骨密度。

Abstract: Background: The series of symptoms caused by decreased bone density seriously affect human quality of life. Therefore, it is of great significance to explore the causal relationship between tea intake and bone density for health. Objective: To explore the causal relationship between tea intake and total body bone mineral density using a two sample Mendelian randomization method. Method: Genome wide association analysis data on tea intake and whole body bone density were obtained from the publicly available GWAS database website. The causal relationship between tea intake and whole body bone density was evaluated using two sample Mendelian randomization methods such as weighted median, inverse variance weighted, and MR Egger analysis, Finally, sensitivity analysis is applied to evaluate whether the conclusion is reliable. Result: IVW inverse variance weighting method (OR = 1.204308, 95% CI: 1.0620635~1.365603, P = 0.003744053), MR Egger analysis (OR = 1.062008, 95% CI: 0.7962864~1.416400, P = 0.684541424), weighted median method (OR = 1.178897, 95% CI: 0.9937853~1.398488, P = 0.058963289); Heterogeneity test (IVW Q-pval = 0.11, MR Egger Q-pval = 0.12), pleiotropy test (pval = 0.3474897). Conclusion: Mendelian randomization results showed a causal relationship between tea intake and the risk of systemic bone density, and drinking tea helped improve systemic bone density.