普朗尼克F127调控下MnFe₂O₄纳米粒子的共沉淀制备及其性能表征

Pluronic F127 Regulated Coprecipitation Preparation and Characterization of MnFe₂O₄ Nanoparticles

摘要: MnFe₂O₄纳米粒子在核磁共振成像和肿瘤磁热疗的应用上具有很大潜力。但是其应用于生物医学领域的主要障碍是难以制备形貌均一、分散性好、磁性能优异且生物相容性良好的MnFe₂O₄纳米粒子。目的：通过F127调控下的共沉淀法制备粒径均匀且磁性能较好的MnFe₂O₄纳米粒子，且通过普朗尼克F127的修饰提高其分散性并降低MnFe₂O₄纳米粒子的细胞毒性。方法：以F127为模版，采用共沉淀法制备MnFe₂O₄磁性纳米粒。利用X射线衍射(XRD)、透射电子显微镜(TEM)、振动样品磁强计(VSM)、傅立叶变换红外光谱(FT-IR)等对样品的成分、微观结构、形貌和粒径以及磁性能进行表征。通过MTT实验对样品的细胞毒性进行评价。结果：采用普朗尼克F127调控下的共沉淀法成功制备大约50 nm的球形MnFe₂O₄铁磁性纳米粒子，其比饱和磁化强度为44.8 emu/g，对HUVEC细胞无明显毒性。结论：共沉淀过程中，F127能够控制MnFe₂O₄纳米粒子的形貌、粒径，从而提高其磁性能，另外还可以降低其细胞毒性。该方法在生物医学应用上具有很大的潜力。

Abstract: MnFe₂O₄ nanoparticles have great potentials in Magnetic Resonance Imaging and tumor hyper-thermia. However for biomedical applications, the main challenges would be of great difficulty to synthesize MnFe₂O₄ nanoparticles with homogeneous size and morphology, high magnetization, and good biocompatibility. Pluronic F127 was utilized to regulate the coprecipitation process of MnFe₂O₄ nanoparticles. Objective: The regulation of Pluronic F127 in the coprecipitation process was expected to facilitate the formation of MnFe₂O₄ nanoparticles with uniform size and morphology, higher magnetization, and low cytotoxicity. Methods: MnFe₂O₄ nanoparticles were prepared by chemical coprecipitation method in presence of Pluronic F127. X-ray diffraction (XRD), transmission electron microscopy (TEM), and vibrating sample magnetometer (VSM) were used to characterize the composition, microstructure, morphology, and magnetization of the prepared MnFe₂O₄ nanoparticles. And MTT assays were conducted to investigate their cytotoxicity. Results: The ferromagnetic MnFe₂O₄ nanoparticles (F127-NPs) were successfully synthesized in the presence of Pluronic F127 via coprecipitation method. Compared to the nanoparticles prepared without Pluronic F127, F127-NPs exhibited a more uniform size of ca. 50 nm and a sphere-like shape. Moreover, F127-NPs possessed a higher magnetization (44.8 emu/g) and exhibited little inhibition to HUVE cells. Conclusion: Pluronic F127 could regulate the coprecipitation process to form the high quality MnFe2O4 nanoparticles and improve the cytocompatibility of nanoparticles. And this method is of a great potential to be applied in the field of biomedicine.