Au@1.66/Bi2MoO6基夹心型双循环电化学免疫传感器用于CA125的超灵敏检测
Ultrasensitive Detection of CA125 Using an Au@CeO1.66/Bi2MoO6-Based Sandwich-Type Electrochemical Immunosensor with Dual Cycling
摘要: 肿瘤早期精准检测是当前物理化学与临床医学交叉领域的研究热点，糖类抗原125 (CA125)作为卵巢癌等妇科恶性肿瘤的特异性标志物，其高灵敏检测对肿瘤筛查与病程监测具有重要意义。本研究设计合成Au@GO-Chi-Fc与Au@CeO1.66/Bi2MoO6铋基复合纳米钙钛矿材料，基于电化学–化学–化学(ECC)双循环信号放大策略，构建夹心型电化学免疫传感器用于CA125的超灵敏检测。通过TEM、SEM、XPS、XRD等表征手段完成复合纳米材料的形貌、元素组成与物化性能分析，验证了铋基钙钛矿材料优异的生物相容性、结构稳定性与电化学活性。测试结果表明，该免疫传感器在宽浓度范围内对CA125表现出优异的选择性、重复性与长期稳定性，检测限达到极低水平。本工作为肿瘤标志物的高灵敏电化学检测提供了新的材料体系与构建思路，拓展了铋基钙钛矿材料在生物传感领域的应用场景。
Abstract: Early and accurate detection of tumors is a research hotspot in the interdisciplinary field of physical chemistry and clinical medicine. Carbohydrate Antigen 125 (CA125), as a specific biomarker for gynecological malignant tumors such as ovarian cancer, its highly sensitive detection is of great significance for tumor screening and disease course monitoring. In this study, bismuth-based composite nano-perovskite materials Au@GO-Chi-Fc and Au@CeO1.66/Bi2MoO6 were designed and synthesized. Based on the Electrochemistry-Chemistry-Chemistry (ECC) dual-cycle signal amplification strategy, a sandwich-type electrochemical immunosensor was constructed for the ultra-sensitive detection of CA125. The morphology, elemental composition and physicochemical properties of the composite nanomaterials were analyzed through characterization methods including TEM, SEM, XPS and XRD, which verified the excellent biocompatibility, structural stability and electrochemical activity of the bismuth-based perovskite materials. The test results show that the immunosensor exhibits excellent selectivity, repeatability and long-term stability for CA125 in a wide concentration range, with an extremely low detection limit. This work provides a new material system and construction idea for the highly sensitive electrochemical detection of tumor biomarkers, and expands the application scenarios of bismuth-based perovskite materials in the field of biosensing.
文章引用：封美娜. Au@1.66/Bi2MoO6基夹心型双循环电化学免疫传感器用于CA125的超灵敏检测[J]. 物理化学进展, 2026, 15(4): 301-314. https://doi.org/10.12677/japc.2026.154029

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