血必净对脓毒症大鼠皮质酮及肾上腺组织GRmRNA水平的影响

Xuebijing Regulate the Hypothalamic-Pituitary-Adrenal Axis and the Level of GRmRNA of Adrenal in Septic Rats

摘要: 目的：探讨在血必净干预下，脓毒症大鼠血浆皮质酮(CORT)水平以及肾上腺组织糖皮质激素受体(GR mRNA)水平的变化，明确血必净对脓毒症大鼠CORT及GR mRNA的调节作用。方法：选用健康雄性SD大鼠40只，随机分为正常对照组(Normal group)，假手术组(Sham group)，模型组(Sepsis group)和血必净治疗组(XBJ group)四个组，采用经典的盲肠结扎穿孔法(CLP)制作SD大鼠脓毒症模型。CLP术后6小时处死动物，利用放射免疫法检测血浆皮质酮(CORT)水平；RT-PCR法检测肾上腺组织GRmRNA水平。结果：Sepsis组血浆CORT含量(29.74 ± 1.65 ng/ml)明显高于Normal组(7.56 ± 0.89 ng/ml, P < 0.01)及Sham组(13.27 ± 1.31 ng/ml, P < 0.01)；XBJ组血浆CORT含量(19.27 ± 1.85 ng/ml)明显低于Sepsis组(P < 0.01)；Normal组与Sham组之间相比无统计学差异(P > 0.05)；Sepsis组肾上腺组织GR mRNA含量(0.80 ± 0.11)明显低于Normal组(1.44 ± 0.07, P < 0.01)和Sham组(1.15 ± 0.11, P < 0.01)；XBJ组肾上腺组织GR mRNA含量(1.08 ± 0.10)和Sepsis组之间相比无统计学差异(P > 0.05)。结论：脓毒症早期大鼠血浆CORT的水平是升高的，而肾上腺组织GRmRNA水平是较低的；血必净干预后能够降低血浆CORT的水平并且一定程度上能够提高肾上腺组织GRmRNA的水平，减轻脓毒症大鼠的过度应激状态，改善脓毒症大鼠的感染中毒症状。

Abstract: Objective: To investigate the change of CORT and the level of GRmRNA of adrenal in CLP induced septic rats which were treated by Xuebijing, identify that Xuebijing can regulate the CORT and GRmRNA. Methods: 40 male rats were divided randomly into normal control group, sham group, saline group (Sepsis group), Xuebijing for treatment group. The model of sepsis was made by CLP. We observe the clinical manifestation of animal after CLP. We use the radio-immunity method to detect the levels of CORT; using the RT-PCR method to measure the level of GRmRNA in adrenal. Results: In Sepsis group, the plasma CORT concentration (29.74 ± 1.65 ng/ml) was significantly higher than that in the normal control group (7.56 ± 0.89 ng/ml, P < 0.01) and Sham group (13.27 ± 1.31 ng/ml, P < 0.01). In XBJ group, plasma CORT concentration (19.27 ± 1.85 ng/ml) was significantly lower than that in Sepsis group (P < 0.01). There was no significant difference between Normal control and Sham group (P > 0.05). In Sepsis group, the level of the GRmRNA in the adrenal tissue (0.80 ± 0.11) was significantly lower than that in the normal control group (1.44 ± 0.07, P < 0.01) and the Sham group (1.15 ± 0.11, P < 0.01). There was no significant difference between XBJ (1.08 ± 0.10) and Sepsis group (P > 0.05). Conclusion: In septic rats, the HPA axis was excessive activate. Xuebijing can decrease the level of CORT, increase the level of GRmRNA in adrenal, improve the excessive activate condition of HPA axis, and lessen the clinical manifestation.