电喷雾质谱法快速分析三种农药的残留

Rapid Analysis of Three Kinds of Pesticide Residues Using Electrospray Ionization Mass Spectrometry

摘要: 本文采用电喷雾电离质谱技术(Electrospray ionization mass spectrometry, ESI-MS)并结合源内碰撞解离质谱技术(In-source collision induced dissociation, In-source CID)，对三种常见农药5%己唑醇微乳剂、30%吡虫啉微乳剂和40%苯醚甲环唑悬浮剂的化学成分进行了分析。被测样品经过溶解、稀释、过滤后，直接进行质谱分析。检测方法采用电喷雾电离离子源(ESI)，5%己唑醇微乳剂样品采用负离子模式检测，30%吡虫啉微乳剂样品采用正负两种离子模式检测，40%苯醚甲环唑悬浮剂样品采用正离子模式检测。通过电喷雾电离质谱技术并结合源内碰撞解离质谱技术，分别得到了5%己唑醇微乳剂、30%吡虫啉微乳剂和40%苯醚甲环唑悬浮剂样品主要成分的分子离子和多级串联碎片离子，并分析了其可能的质谱断裂规律。该方法样品需求量少，方法简单、分析速度快，适用于农药以及农药残留的分析，并为5%己唑醇微乳剂、30%吡虫啉微乳剂和40%苯醚甲环唑悬浮剂三种农药作用机制的研究奠定了基础。

Abstract: Electrospray ionization mass spectrometry (ESI-MS) combined with electrospray ionization tandem mass spectrometry technology was used for rapid analysis of three kinds of pesticide residues. In this manuscript, the rapid analytical method for Hexaconazo, Iimidacloprid residues, as well as the rule of tandem mass spectrometry for Difenconazole, was established. The method is simple and rapid. It is applicable to determine the pesticide and pesticide residues.