一株藜内生放线菌的活性分析及抗生素生物合成潜力的筛查
Activity Analysis and Antibiotics Biosynthesis Potential Screening of an Endophytic Actinomycetes from Chenopodium album L.
DOI: 10.12677/AMB.2016.52002, PDF,    科研立项经费支持
作者: 甘龙站, 王 幸, 刘 杨, 田永强*：四川大学轻纺与食品学院，皮革化学与工程教育部重点实验室，四川 成都
关键词: 藜；内生放线菌；鉴定；活性；抗生素合成酶基因；Chenopodium album L.； Endophytic Actinomycetes； Identification； Activity； Antibiotics Synthetase Genes
摘要: 对一株分离自药用植物藜的内生放线菌LCB-A236进行系统学鉴定。电镜扫描后观察到螺旋状的孢子丝和椭球形的孢子，通过其形态和生理生化特征初步确定为链霉菌。分子鉴定和系统发育分析结果显示，其与Streptomyces violaceorubidus LMG 20319T (AJ781374)进化地位最接近，相似度为99.71%，确定为Streptomyces sp.。通过平板法、SRB法和Lilian Sauma等人的激动率测试模型进行抗菌、抗癌及抗糖尿病活性测试，结果显示出较强的抗癌及抗糖尿病活性和中等的抗菌活性。菌株的5类抗生素合成酶基因PCR扩增筛查结果表明，该菌株含有I-型聚酮合成酶、II-型聚酮合成酶和非核糖体多肽合成酶的基因。
Abstract: An endophytic actinomycetes strain LCB-A236 isolated from medicinal plant Chenopodium al-bum L. was identified systematically. The strain produced spiral sporotrichial and ellipsoidal spores according to microscopic observation. On the basis of morphological, physiological and biochemical characteristics, the strain was identified as a member of genus Streptomyces. Phy-logenetic analysis indicated that the strain exhibited the highest similarity (99.71%) to Strep-tomyces violaceorubidus LMG 20319T (AJ781374). We confirmed it as Streptomyces sp. According to the results of activity measurements by plate method, SRB bacteriostasis and Lilian Sauma et al.’s test model of activation rate, it previously described that the strain demonstrated strong anticancer, antidiabetes activities and medium antimicrobial activity. PCR screening for the genes of five kinds of antibiotic synthetase showed that the strain possessed the genes of polyketides synthetase I, polypeptide synthetase II, and non-ribosome polypeptide synthetase simultaneously.
文章引用：甘龙站, 王幸, 刘杨, 田永强. 一株藜内生放线菌的活性分析及抗生素生物合成潜力的筛查[J]. 微生物前沿, 2016, 5(2): 9-18. https://dx.doi.org/10.12677/AMB.2016.52002

参考文献

[1] Herre, E.A., Mejia, L.C., Kyllo, D.A., Rojas, E., Maynard, Z., et al. (2007) Ecological Implications of Anti-Pathogen Effects of Tropical Fungal Endophytes and Mycorrhizae. Ecology, 88, 550-558. http://dx.doi.org/10.1890/05-1606 [Google Scholar] [CrossRef] [PubMed]
[2] Qin, S., Xing, K., Jiang, J.H., Xu, L.H. and Li, W.J. (2010) Biodiversity, Bioactive Natural Products and Biotechnological Potential of Plant-Associated Endophytic Actinobacteria. Applied Microbiology and Biotechnology, 89, 457- 473. http://dx.doi.org/10.1007/s00253-010-2923-6 [Google Scholar] [CrossRef] [PubMed]
[3] Ezra, D., Castillo, U., Strobel, G.A., Hess, W.M., Porter, H., et al. (2004) Coronamycins, Peptide Antibiotics Produced by Averticillate Streptomyces sp. (MSU-2110) Endophytic on Monstera sp. Microbiology, 150, 785-793. http://dx.doi.org/10.1099/mic.0.26645-0 [Google Scholar] [CrossRef] [PubMed]
[4] Igarashi, Y., Trujillo, M.E., Martnez-Molina, E., Yanase, S., Miyanaga, S., et al. (2007) Antitumor Anthraquinones from an Endophytic Actinomycete Mieromonospora lupini sp. nov. Bioorganic & Medicinal Chemistry Letters, 17, 3702-3705. http://dx.doi.org/10.1016/j.bmcl.2007.04.039 [Google Scholar] [CrossRef] [PubMed]
[5] 郭泽经, 冯治翔, 吴华动, 等. 1株罗汉杉内生放线菌的鉴定、活性分析及抗生素生物合成基因的筛查[J]. 微生物学杂志, 2011, 31(6): 23-25.
[6] Qiu, P., Feng, Z.X., Tian, J.W., Lei, Z.C., Wang, L., et al. (2015) Diversity, Bioactivities, and Metabolic Potentials of Endophytic Actinomycetes Isolated from Traditional Medicinal Plants in Sichuan, China. Chinese Journal of Natural Medicines, 13, 942-953. http://dx.doi.org/10.1016/S1875-5364(15)30102-3 [Google Scholar] [CrossRef] 
[7] 徐丽华, 李文均, 刘志恒, 等. 放线菌系统学[M]. 北京: 科学出版社, 2007: 40-46.
[8] Gauthier, M.J., Shewan, J.M., Gibson, D.M. and Lee, J.V. (1975) Taxonomic Position and Seasonal Variations in Marine Neritic Environment of Some Gram-Negative Antibiotic-Producing Bacteria. Journal of General Microbiology, 87, 211-218. http://dx.doi.org/10.1099/00221287-87-2-211 [Google Scholar] [CrossRef] [PubMed]
[9] Skehan, P., Storeng, R., Scudiero, D., Monks, A., McMahon, J., et al. (1990) New Colorimetric Cytotoxicity Assay for Anticancer-Drug Screening. Journal of the National Cancer Institute, 82, 1107-1112. http://dx.doi.org/10.1093/jnci/82.13.1107 [Google Scholar] [CrossRef] [PubMed]
[10] Sauma, L., Stenkula, K.G., Kjølhede, P., et al. (2006) PPAR-γ Response Element Activity in Intact Primary Human Adipocytes: Effects of Fatty Acids. Nutrition, 22, 60-68. http://dx.doi.org/10.1016/j.nut.2005.04.011 [Google Scholar] [CrossRef] [PubMed]
[11] 徐平, 李文均, 张永光, 等. 产生大环聚酮类天然产物放线菌的分子筛选研究[J]. 中国抗生素杂志, 2003, 28(6): 321-375.
[12] Ayuso-Sacido, A. and Genilloud, O. (2005) New PCR Primers for the Screening of NRPS and PKS-I Systems in Actinomycetes: Detection and Distribution of These Biosynthetic Gene Sequences in Major Taxonomic Groups. Microbial Ecology, 49, 10-24. http://dx.doi.org/10.1007/s00248-004-0249-6 [Google Scholar] [CrossRef] [PubMed]
[13] Wood, S.A., Kirby, B.M., Goodwin, C.M., Strålfors, P., Söderström, M., et al. (2007) PCR Screening Reveals Unex- pected Antibiotic Biosynthetic Potential in Amycolatopsis sp. Strain UM16. Journal of Applied Microbiology, 102, 245-253. http://dx.doi.org/10.1111/j.1365-2672.2006.03043.x [Google Scholar] [CrossRef] [PubMed]
[14] 李文均, 徐平, 徐丽华, 等. 极端环境中的放线菌资源[J]. 微生物学通报, 2003(30): 125-127.
[15] 杜慧娟, 王伯初, 米鹏程, 等. 药用植物内生菌的分离及抗菌活性的初步研究[J]. 氨基酸和生物资源, 2008, 30(1): 61-64.
[16] 郭小炜, 冯俊涛, 易晓华, 等. 大花金挖耳内生菌的分离及抑菌活性筛选[J]. 西北植物学报, 2007, 27(2): 377- 383.
[17] 陈文强, 彭浩, 邓百, 等. 药用植物虎杖内生放线菌的分离及抑菌活性的研究[J]. 陕西理工学院学报: 自然科学版, 2012(6): 61-67.
[18] 冯天祥, 王玲, 陈海敏, 等. 植物内生放线菌功能及生物活性物质研究进展[J]. 中国生物工程杂志, 2015, 35(4): 98-106.
[19] Stierle, A. and Strobel, G. (1993) Taxol and Taxane Production by Taxomyces Andreanae an Endophytic Fungus of Pacific yew. Science, 260, 214. http://dx.doi.org/10.1126/science.8097061 [Google Scholar] [CrossRef] [PubMed]
[20] 杨桂柳, 杨立芳, 姜明国, 等. 北仑河红树林内生放线菌分离、鉴定及活性研究[J]. 农业生物技术学报, 2015, 23(6): 894-904.
[21] 姚晓玲, 康前进, 熊顺子, 等. 喜树种子内生放线菌的分离鉴定及抗菌活性物质初分离[J]. 微生物学通报, 2014, 41(6): 1109-1120.
[22] 刘松艳, 张沐新, 吴月红, 等. 藜中黄酮类化学成分及抑菌效果的研究[J]. 东北师大学报: 自然科学版, 2011, 43(1): 93-96.
[23] 赵良忠, 王放银, 段林东. 灰灰菜抗菌物质提取及抗菌效果研究[J]. 生物技术, 2004, 14(4): 66-67.

为你推荐