磷酸盐对盐生杜氏藻生长和细胞色素积累的影响

Effects of Phosphate on the Growth and Cell Pigment Accumulation of Dunaliella salina

摘要: 研究了不同浓度(0~0.30 mmol/L)的KH₂PO₄对盐生杜氏藻生长和细胞色素积累的影响，结果表明，添加0.10 mmol/L KH₂PO₄，细胞生长最快，最高密度为153 × 10⁴ cell/mL，无磷对照组密度最低，仅为36 × 104 cell/mL；KH₂PO₄浓度为0.20 mmol/L时，β-胡萝卜素积累量最高，为12.8 mg/L，其次是0.15 mmol/L，对照组很低，仅为6.28 mg/L，KH₂PO₄超过0.25 mmol/L对β-胡萝卜素积累有抑制作用；适当添加KH₂PO₄对D. salina叶绿素a合成有促进作用；在培养的前6天，pH值明显上升，随后4~5天又下降；添加0.1 mmol/L KH₂PO₄细胞蛋白质含量最高，为37.34%，对照组仅为24.86%；0.10 mmol/L组的KH₂PO₄在6天内吸收完毕，KH₂PO₄吸收的时间与其最初的浓度呈正相关关系，建立了藻液中KH₂PO₄被盐生杜氏藻吸收的动力学方程。

Abstract: The effects of KH₂PO₄ with different levels on the growth and cell pigment accumulation of Dunaliel-la salina were investigated and the results indicated that the maximum growth of 153 × 104 cell/mL was detected in culture medium with 0.1 mmol/L KH₂PO₄ and the minimum of 36 × 104 cell/mL in the control group. The culture medium with 0.20 mmol/L KH₂PO₄ had the highest β-carotene con-tent of 12.8 mg/L, followed by 11.04 mg/L with 0.15 mmol/L KH₂PO₄, and the control group had lower β-carotene content of only 6.28 mg/L. Over 0.25 mmol/L KH₂PO₄ was found to have depress-ing function to cell growth. Right level KH₂PO₄ was helpful to chlorophyll a biosynthesis. During the first 6 days within culture period, pH values of the culture media rose up, and then declined in the following 4 - 5 days. The cells which were cultured in media with 0.10 mmol/L KH₂PO₄ had the high-est protein content of 37.34%, while the control group only had 24.86%; 0.10 mmol/L KH₂PO₄ was assimilated completely within 6 days, and the uptake time had right-relationship with KH₂PO₄ con-tents. Finally, the assimilated mechanic equation of KH₂PO₄ by D. salina was set up.