大规模开发及特性分析十字花科SSR分子标记及其数据库的构建
Large-Scale Development and Character Analysis of SSR Markers and Database Build in Brassicaceae
DOI: 10.12677/BR.2017.63013, PDF,  被引量    科研立项经费支持
作者: 杨帅*：山东农业大学植物保护学院，山东 泰安；济南大学生命科学院，山东 济南；李慧：济南大学生命科学院，山东 济南；侯欣, 张丽*：山东农业大学植物保护学院，山东 泰安
关键词: 十字花科；SSR；特异引物；通用引物；数据库；Brassicaceae； SSR； Specific Primers； Universal Primers； Database
摘要: 十字花科在植物界中是一种极具价值的科，简单重复序列(Simple Sequence Repeats, SSR)在十字花科的研究中发挥着及其重要的作用。本研究利用已知13个十字花科物种的基因组，借助生物信息学和比较基因组学的方法，获得了1,786,619个SSR位点及1,919,464对SSR引物。结果显示SSR位点广泛分布于十字花科物种的基因组中，其中1~3单元的重复在基因组和基因序列中占有率较高，二单元重复中的AT/TA重复单元的数目占总数目的大部分。利用435,414对物种特异性SSR，可以进行十字花科物种关联分析。11对通用引物的开发，说明了十字花科物种内存在一致性区段，可以进行引物跨物种扩增。本研究构建了世界首个十字花科SSR分子标记数据库平台(BSSRD, Brassicaceae Simple Sequence Repeats Database http://biodb.sdau.edu.cn/BSSRD)，该平台将会在以后十字花科植物的遗传图谱的构建，基因定位和遗传育种中发挥重要的作用。
Abstract: Brassicaceae is an important family in the plant kingdom. The Simple Sequence Repeats (SSRs) play a vital role in the study of Brassicaceae. By using 13 known sequenced Brassicaceae species with bioinformatics and comparative genomics methods, a total of 1,786,619 SSR loci and 1,919,464 pair of primers have been developed. The results show that the SSRs are widely distributed in the Brassicaceae species’ genomes, 1 - 3 bases duplication have a high ratio among these genomes and gene sequences, AT/TA repeats units have a high numbers in all of the 2 base duplication. In addition, 435,414 specific SSR primers could be used to analyze the correlation between the species of Brassicaceae. 11 pairs of universal primers’ developed shows that there exist some consistent base fragments and could be amplified across different species. In this study, we constructed the world’s first SSR molecular marker database platform (BSSRD, Brassicaceae Simple Sequence Repeats Database http://biodb.sdau.edu.cn/BSSRD) which will play an important role in the construction of genetic map, gene mapping and genetic breeding of Brassicaceae.
文章引用：杨帅, 李慧, 侯欣, 张丽. 大规模开发及特性分析十字花科SSR分子标记及其数据库的构建[J]. 植物学研究, 2017, 6(3): 86-95. https://doi.org/10.12677/BR.2017.63013

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