氨氮胁迫对泥蚶免疫酶活性的影响

Effects of Ammonia-N Stress on the Activities of Immune Enzymes of Tegillarca granosa

摘要: 本实验以泥蚶为研究对象，研究了不同浓度下的氨氮胁迫对三种免疫酶活性的影响。实验结果发现：1) 氨氮浓度对泥蚶过氧化氢酶(CAT)活力的影响不显著(F_6,14 = 2.6074, P = 0.06545 > 0.05)，随着氨氮浓度的增加，总体上CAT活性表现出“诱导–抑制”的趋势。2) 氨氮浓度极显著影响泥蚶酸性磷酸酶(ACP)的活力(F_6,14 = 13.693, P = 0.00004 < 0.01)，随着氨氮浓度的增加，总体上ACP活性表现出“抑制–诱导”的趋势。3) 氨氮浓度极显著影响泥蚶碱性磷酸酶(AKP)的活力(F_6,14 = 13.696, P = 0.00004 < 0.01)，随着氨氮浓度的增加，总体上AKP活性表现出“诱导–抑制”的趋势。结果表明，氨氮胁迫影响泥蚶这三种免疫酶的活性。

Abstract: In this paper, the effects of ammonia-N stress on the activities of immune enzyme of Tegillarca granosa were studied at different conditions with ecological method. The results were as follows: 1) Ammonia-N concentration was found to have no significant effect on the activity of catalase (CAT) of Tegillarca granosa (F_{6, 14} = 2.6074, P = 0.06545 > 0.05). As the concentration of ammonia-N increased, CAT generally demonstrated an “induction-suppression” activity. 2) Ammonia-N concentration had a highly significant impact on the activity of acid phosphatase (ACP) of Tegillarca granosa (F_{6, 14} = 13.693, P = 0.00004 < 0.01). As the concentration of ammonia-N increased, ACP generally demonstrated a “suppression-induction” activity. 3) Ammonia-N concentration had a highly significant impact on the activity of alkaline phosphatase (AKP) of Tegillarca granosa (F_{6, 14} = 13.696, P = 0.00004 < 0.01). As the concentration of ammonia-N increased, AKP generally demonstrated an “induction-suppression” activity. In conclusion, the stress of ammonia-N could influence these immuno-enzymes’ activities.