珍珠层可溶性蛋白对酪氨酸酶的作用机理研究
Study of Tyrosinase Inhibition Activities of Nacre Soluble Proteins
DOI: 10.12677/AMB.2017.64014, PDF,    科研立项经费支持
作者: 程 卓, 刘春平, 卢延斌*：浙江工商大学海洋食品研究院，浙江 杭州
关键词: 珍珠；可溶性蛋白；酪氨酸酶抑制作用；机理；Pinctada fucata； Soluble Proteins； Tyrosinase Inhibition Activity； Mechanism
摘要: 研究了珍珠层可溶性蛋白对酪氨酸酶的抑制作用、抑制类型及抑制机理。实验结果表明，该蛋白对酪氨酸酶有良好的抑制效果，且样品浓度与酶活力之间存在剂量依赖关系。珍珠层可溶性蛋白能显著延长酪氨酸酶单酚酶催化反应的迟滞时间；对二酚酶的抑制类型为可逆抑制，抑制机理是竞争性抑制。
Abstract: Nacre proteins were evaluated for their inhibition on tyrosinase. The results showed that it had good inhibition on tyrosinase. It also demonstrated that nacre proteins can significantly extend the lag time of the reaction, which was catalyzed by monophenolase of tyrosinase. The effect of nacre proteins on the diphenolase of tyrosinase is reversible inhibition of competitive inhibition.
文章引用：程卓, 刘春平, 卢延斌. 珍珠层可溶性蛋白对酪氨酸酶的作用机理研究[J]. 微生物前沿, 2017, 6(4): 108-115. https://doi.org/10.12677/AMB.2017.64014

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