药用大黄染色体的核型分析
Karyotype Analysis of Rheum officinale Chromosome
DOI: 10.12677/BR.2018.71001, PDF,    国家自然科学基金支持
作者: 黄 敏, 曾丹丹, 李文丽, 宋 杰, 王戌梅:西安交通大学,药学院,陕西 西安
关键词: 药用大黄倍性染色体核型分析Rheum officinale Ploidy Chromosome Karyotype Analysis
摘要: 对药用大黄(Rheum officinale Baill.)的染色体核型进行研究。采用常规根尖压片法分析染色体特征,并利用显微摄影技术对染色体进行分析。结果表明,药用大黄的核型公式为2n = 2x = 22 = 4M + 18m,核型类型为“1A”,核型不对称系数(As.K.%)为54.81%。染色体倍性为二倍体,不存在多倍化现象。药用大黄染色体的数量稳定,形态清晰,可为进一步深入研究奠定基础。
Abstract: The karyotype of Rheum officinale was analyzed in the present study. The chromosome features were conducted by conventional root-tip squashing method and photomicrography technique. Results were as follows: The karyotype formula of R. officinale from Luanchuan in Henan was 2n = 2x = 22 = 18m + 4M; karyotype was 1A type; karyotype asymmetry index (As.K.%) was 54.81%. Chromosome ploidy was diploid, and no polyploidy was observed. The chromosome number and the morphology of R. officinale are stable and clear, which can provide the foundation for further research.
文章引用:黄敏, 曾丹丹, 李文丽, 宋杰, 王戌梅. 药用大黄染色体的核型分析[J]. 植物学研究, 2018, 7(1): 1-6. https://doi.org/10.12677/BR.2018.71001

参考文献

[1] 国家药典委员会. 中国药典: 2015年版: 一部 [S]. 北京: 中国医药科技出版社, 2015: 23-24.
[2] Bao, B.J. and Grabovskaya-Borodina, A.E. (2003) Rheum. In: Li, A.R. and Bao, B.J., Eds., Flora of China, Volume 5, Science Press, Beijing; Missouri Botanical Garden, St. Louis, 341-350.
[3] 郑俊华, 西泽信, 山岸乔, 西冈五夫, 金子光, 三桥博, 楼之岑. 正品大黄中34种化学成分的高效液相色谱法定量分析[J]. 北京大学学报, 1989(1): 315-317.
[4] Shang, X. and Yuan, Z. (2003) Determination of Active Components in Rhubarb and Study of Their Hydrophobicity by Micellar Electrokinetic Chromatography. Bioorganic & Medicinal Chemistry Letters, 13, 617-622.
[Google Scholar] [CrossRef
[5] 王家葵, 李傲, 王慧, 徐晓玉. 正品大黄不同品种间泻下效价强度比较研究[J]. 中国中药杂志, 2006, 31(23): 1987-1991.
[6] Liu, L., Guo, Z.J., Lv, Z.G., et al. (2008) The Beneficial Effect of Rheum tanguticum Polysaccharide on Protecting against Diarrhea, Colonic Inflammation and Ulceration in Rats with TNBS-Induced Colitis: The Role of Macrophage Mannose Receptor in Inflammation and Immune Response. International Immunopharmacology, 8, 1481-1492.
[Google Scholar] [CrossRef] [PubMed]
[7] 高亮亮, 许旭东, 南海江, 杨俊山, 陈士林. 唐古特大黄化学成分研究[J]. 中草药, 2011, 42(3): 443-446.
[8] Sun, M., Li, L., Mei, W., et al. (2016) Effects of Growth Altitude on Chemical Constituents and Delayed Luminescence Properties in Medicinal Rhubarb. Journal of Photochemistry and Photobiology B: Biology, 162, 24-33.
[Google Scholar] [CrossRef] [PubMed]
[9] Liu, R.R., Wang, A.L., Tian, X.M., et al. (2010) Uniformity of Karyotypes in Rheum (Polygonaceae), a Species-Rich Genus in the Qinghai-Tibetan Plateau and Adjacent Regions. Caryologia, 63, 82-90.
[Google Scholar] [CrossRef
[10] 胡延萍, 谢小龙, 温泉, 赵旭东, 王莉, 李毅. 唐古特大黄五个居群的核型[J]. 云南植物研究, 2007, 29(4): 429-433.
[11] Ye, J.Q., Jia, Y.Y., Fan, K., et al. (2014) Kary-otype Analysis of Rheum palmatum. Genetics and Molecular Research, 13, 9056-9061.
[Google Scholar] [CrossRef
[12] Jaretzky, R. (1928) Histologische und karyologische studien an Polygonaceen. Jahrbucher fur Wissenschaftliche Botanik, 69, 357-490.
[13] Stebbins, G.L. (1971) Chromosomal Evolution in Higher Plants. Edward Aronld Ltd., London, 87-93.
[14] 洪德元. 植物细胞分类学[M]. 北京: 科学出版社, 1990: 1-11.
[15] Stace, C.A. (2000) Cytology and Cytogenetics as a Fundamental Taxonomic Resource for the 20th and 21st Centuries. Taxon, 49, 451-477.
[Google Scholar] [CrossRef
[16] Wang, X.M., Hou, X.Q., Zhang, Y.Q., et al. (2010) Distribution Pattern of Genuine Species of Rhubarb as Traditional Chinese Medicine. Journal of Medicinal Plants Research, 4, 1865-1876.
[17] Wang, X.M., Hou, X.Q., Zhang, Y.Q., et al. (2012) Genetic Diversity of the Endemic and Medicinally Important Plant Rheum officinale as Revealed by Inter-Simpe Sequence Repeat (ISSR) Markers. International Journal of Molecular Sciences, 13, 3900-3915.
[Google Scholar] [CrossRef] [PubMed]
[18] Wang, X.M., Yang, R., Feng, S.F., et al. (2012) Genetic Variation in Rheum palmatum and Rheum tanguticum (Polygonaceae), Two Medicinally and Endemic Species in China Using ISSR Markers. PLoS ONE, 7, e51667.
[Google Scholar] [CrossRef] [PubMed]
[19] Wang, X.M., Hou, X.Q., Zhang, Y.Q., et al. (2014) Morpho-logical Variation in Leaf Dissection of Rheum palmatum Complex (Polygonaceae). PLoS ONE, 9, e110760.
[Google Scholar] [CrossRef] [PubMed]
[20] 李懋学, 张赞平. 作物染色体及其研究技术[M]. 北京: 中国农业出版社, 1996: 23-37.
[21] 李懋学, 陈瑞阳. 关于植物核型分析的标准化问题[J]. 武汉植物学研究, 1985, 3(4): 297-302.
[22] Arano, H. (1963) Cytological Studies in Subfamily Carduoideae (Compositae) of Japan, IX. The Karyotype Analysis and Phylogenetic Consideration on Pertya and Ainsliaea. Botanical Magazine (Tokyo), 76, 32-39.
[Google Scholar] [CrossRef
[23] Levan, A., Fredga, K. and Sandberg, A. (1964) Nomenclature for Centromeric Position on Chromosomes. Hereditas, 52, 201-220.
[Google Scholar] [CrossRef
[24] Kuo, S.R., Wang, T.T. and Huang, T.C. (1972) Karyo-type Analysis of Some Formosan Gymnosperms. Taiwania, 17, 66-80.