临产前后胎盘上bcl-2、Fasl的表达与母血、羊水中ET-1、PGE₂浓度之间的关系

A Relationship between the Expressions of bcl-2 and Fasl in Placental and the Concentrations of ET-1 and PGE₂ in Maternal Blood and Amniotic Fluid on Labor Onset

摘要: 目的：探讨临产前后bcl-2、Fasl在胎盘滋养层细胞的蛋白表达变化及其与母血、羊水中内皮素-1 (Endothelin-1, ET-1)、前列腺素E₂ (ProstaglandinE₂, PGE₂)的浓度之间的关系。方法：53例足月妊娠妇女分成两组，其中临产组28例，未临产组25例。用免疫组化(Immunohistochemical, IHC)法测定胎盘滋养层细胞bcl-2、Fasl的蛋白表达，用放射免疫法测定孕妇母血、羊水中ET-1、PGE₂的浓度。结果：1) 临产组胎盘滋养层细胞bcl-2、Fasl的蛋白表达明显低于未临产组，两组平均灰度及阳性细胞率比较，差异有显著性(p < 0.05)。2) 临产组母血、羊水中ET-1、PGE₂水平均高于未临产组(p < 0.05)。3) 母血、羊水中ET-1的浓度与PGE2的浓度变化呈明显的正相关；母血、羊水中ET-1、PGE₂的变化与bcl-2的蛋白表达呈负相关(p < 0.05)，而与Fasl的表达不相关(p > 0.05)。结论：bcl-2、Fasl介导的胎盘滋养层细胞凋亡在启动分娩发动过程中具有重要作用；bcl-2的作用可能受ET-1、PGE₂等细胞因子的调控。

Abstract: Objective: To investigate the expressions of bcl-2 and Fasl proteins in placental trophoblastic cells on labor onset and their relationships with the concentrations of Endothelin-1 and Prostaglandin E₂ in maternal blood and amniotic fluid. Methods: Fifty-three women on term pregnancy were divided into two groups. One group on the time of labor onset was twenty-eight women. Another group not on the time of labor onset was twenty-five women. Expressions of bcl-2 and Fasl proteins in placental trophoblastic cells were detected by immunohistochemical assay. The concentrations of ET-1 and PGE₂ in maternal blood and amnionotic fluid were detected by radioimmune assay. Results: 1) Expressions of bcl-2 and Fasl proteins in the group of labor onset were significantly lower than that in the group of not labor onset. There was a statistically difference in the average gray scale and the ratio of positive cells between the two groups (p < 0.05). 2) The concentrations of ET-1 and PGE₂ in maternal blood and amniotic fluid of labor onset group were higher than that of not labor onset group (p < 0.05). 3) There was a positive correlation between the concentrations of ET-1 and PGE₂ in maternal blood and amniotic fluid, and the expression of bcl-2 proteins had a negative correlation with the concentrations of ET-1 and PGE₂ respectively, but there was no correlation between the expression of Fasl proteins and the concentrations of ET-1 and PGE₂. Conclusions: It was considered that the apoptosis of placental trophoblastic cells induced by bcl-2 and Fasl may play an important role in the initiation of term labor onset. ET-1 and PGE₂ may control the expression of bcl-2.