雌二醇E2通过调控PEDF表达促进乳腺癌增殖作用研究

The Effect of Estradiol E2 on the Proliferation of Breast Cancer by Regulating PEDF Expression

摘要: 目的：探讨雌二醇E2通过调控色素上皮细胞衍生因子PEDF促进乳腺癌增殖的分子机制。方法：ELISA法检测血清中PEDF水平；放射免疫分析法检测雌二醇E2水平；Western blot检测雌二醇对PEDF调控作用；分子克隆过表达PEDF研究其在雌二醇E2促进乳腺癌增殖中作用；MTT法检测乳腺癌细胞株MCF-7的增殖能力。结果：乳腺癌中PEDF表达下调，且与雌二醇E2水平呈负相关(R = −0.69; ***P < 0.001)；雌二醇E2可下调PEDF的表达，促进乳腺癌细胞MCF-7的增殖。结论：雌二醇E2通过调控PEDF表达进而促进乳腺癌的增殖。

Abstract: Objective: To investigate the molecular mechanism of estradiol E2 to promote the proliferation of breast cancer by regulating the expression PEDF. Methods: Serum PEDF level was detected by ELISA kit and estradiol E2 level was detected by radioimmunoassay; the effect of estradiol on the PEDF regulation was detected by Western blot; the proliferation of breast cancer cell line MCF-7 was detected by MTT assay. Results: The expression of PEDF was down regulated in breast cancer, and negatively correlated with estradiol E2 level. Estradiol E2 can reduce the expression of PEDF and promote the proliferation of breast cancer cell MCF-7. Conclusion: Estradiol E2 can promote the proliferation of breast cancer by regulating the expression of PEDF.