记忆基因(Rab3A)的系统发育分析

Phylogenetic Analysis of Rab3A Gene

摘要: Rab3A是动物大脑中丰度最高的Rab蛋白，而Rab蛋白通过胞吞和胞吐的方式在囊泡运输中起重要作用，由此可见，Rab3A基因对于调控动物的记忆有很重要的作用。从NCBI数据库下载原始核苷酸序列，运用Blast+同源性比对、共线性分析软件ColinearScan、MCscanX进行共线性分析，用MEGA7.0软件中Maximum Likelihood法，Neighbor-joining法分别构建进化树，由于从原始数据进行同源性、共线性分析比对，所以得到了一致性的系统发育树。Rab3A基因的系统发育树与物种发育树一致，由此说明该基因的进化是随着物种的分歧一同发生的，是一种相对古老的基因。

Abstract: Rab3A is the most abundant Rab protein in the animal’s brain. Rab proteins play an important role in vesicular trafficking by exocytosis and endocytosis . Thus, the Rab3A gene plays an important role in the regulation of animal memory. The original nucleotide sequence was downloaded from the NCBI database. Collinearity analysis was carried out by using Blast + homology alignment and ColinearScan and MCscanX. Using MEGA7.0 software Maximum Likelihood method, Neighbor-joining method to construct phylogenetic tree, due to the homology and collinearity analysis and comparison from the original data, a consistent phylogenetic tree was obtained. The phylogenetic tree of Rab3A gene is consistent with the species developmental tree. This shows that the evolution of the gene occurs along with the divergence of species, which is a relatively ancient gene.