小麦PAL蛋白的提取、纯化及活性测定

Extraction, Purification and Activity Determination of Wheat PAL Protein

摘要: 在本研究中，我们构建了PAL基因家族在9种陆地植物中的系统发育树，发现小麦PAL基因家族发生了明显的基因扩张：在这9种陆地植物中，小麦PAL基因家族成员数量最多，达到26个。基因表达模式热图显示，相对于成熟组织，小麦PAL基因在幼嫩组织中的表达量较高。高纯度的小麦PAL蛋白从小麦嫩叶中提取和纯化，并对其进行了活性测定。本研究为小麦PAL基因家族的进一步研究提供了技术支持。

Abstract: In this study, we constructed the phylogenetic tree of PAL gene family in nine land plants, and found that a significant gene expansion occurred in the wheat PAL gene family. Among these nine land plants, the number of wheat PAL genes was the largest, reaching twenty-six. The gene expression pattern showed that the expression level of PAL genes was higher in young tissues than mature tissues. The high purity wheat PAL protein was extracted and purified from young leaves of wheat, and the activity of wheat PAL protein as determined. This study provides technical support for further research on the wheat PAL gene family.