基于Box-Behnken设计–响应曲面法优化丹皮酚的提取工艺

Optimization of Extract Process of Paeonol by Response Surface Method Based on Box-Behnken Design

摘要: 目的：基于Box-Behnken设计–响应曲面法优化丹皮酚的提取工艺。方法：根据单因素实验数据，选择乙醇浓度(体积分数)、提取温度和提取时间，进行三因素三水平的Box-Behnken中心组合试验设计，采用响应曲面法(RSM)分析三个因素对响应值的影响。结果：最佳提取工艺条件为乙醇浓度77.5%，提取温度50℃，提取时间2 h，料液比30:1 (mL/g)时，提取率为92.3%；结论：该丹皮酚提取工艺稳定可靠，能应用于工业化生产。

Abstract: Objective: To optimize the extract process of paeonol from the roots of Paeonia suffruticosa Andrews adopted by response surface method basing on Box-Behnken design. Method: According to the results of the single factor experiment, a three-factor and three-level Box-Behnken central composite design coupled with response surface analysis (RSM) was employed to evaluate the influence of ethanol concentration, extract temperature and time on extract yield of paeonol. Result: The best extract condition was that 77.5% ethanol (V/V) extracted the crude powder of the root of Paeonia suffruticosa Andrewsat 50˚C for 2 h, solid/liquid ratio 30:1 (mL/g), 92.3% extract yield was obtained. Conclusion: The extract process of paeonolis stable and available for further industrial application.