一株腐败希瓦氏菌(Shewanella putrefaciens)培养基优化

Medium (CM0847) Optimization of Shewanella putrefaciens

摘要: 目的：对发酵培养基(CM0847)进行优化促进腐败希瓦氏菌(Shewanella putrefaciens)生长。方法：通过测定菌体在600 nm处的吸光值和菌体干重，采用单因子试验结合正交试验对培养基(CM0847)进行优化。结果：当pH为7.5，在原培养基的基础上葡萄糖、牛肉膏、氯化钠分别以10%、30%及30%的添加量，菌体密度及菌体干重最高。结论：腐败希瓦氏菌在优化后的培养基中培养，其菌体干重较原培养基(CM0847)增加了11.1168 g/L。

Abstract: Aim: The culture medium of Shewanella putrefaciens was optimized and its growth was promoted. Methods: Single factor and orthogonal design experiment were conducted to determine the optimal medium of Shewanella putrefaciens by measured cell density (under 600 nm) and cell weight. Results: Compared with medium CM0847, when the initial pH was 7.5, the glucose, beef extract and sodium chloride added at 10%, 30% and 30%, respectively, the cell density and cell weight were the highest. Conclusion: Cultivated Shewanella putrefaciens in the optimum medium and CM0847 culture medium, the cells weight was improved by 11.1168 g/L.