改性壳聚糖基纳米银复合材料的制备与抗菌性能

Synthesis and Anti/Bacteriostasis of Nano-Silver Composites Based Modified Chitosan

摘要: 目的：制备由NVP改性的壳聚糖(CTS-g-PVP)，以其为模版剂合成聚合物基纳米银复合材料，通过抗/抑菌性和生物相容性实验评价其用作化妆品防腐败剂的可能性。方法：采用原位法合成改性壳聚糖基纳米银复合材料(Nano-Ag@CTS-g-PVP)，以K-B法探究CTS-g-PVP及其纳米银复合材料的抗/抑菌效果，并通过鸡胚尿囊膜实验评价其生物安全性。结果：在0.75% wt CTS-g-PVP水溶液下合成载银量为1.51% wt的Nano-Ag@CTS-g-PVP，其中准球形纳米银的粒径约20~30 nm。此复合材料不论单独使用还是复配，均显示良好的生物相容性和抗/抑菌效果。结论：刺激性低的Nano-Ag@CTS-g-PVP及其复配体系具有良好的抗/抑菌效果，有望应用于化妆品领域。

Abstract: Objective: To prepare chitosan N-vinylpyrrolidone graft copolymer (CTS-g-PVP) and its composites material loaded Nano-silver particles by using CTS-g-PVP as Template. Through the experiments about anti/bacteriostasis and biocompatibility, the composites materials were evaluated the possi-bility of being used as a cosmetic anticorrosion agent. Methods: Nano-Ag@CTS-g-PVP was synthesized using in situ method, and explored the antibacterial effect of it’s by K-B Method. The biosafety was also evaluated by the chick embryo allantoic membrane test. Results: Nano-Ag@CTS-g-PVP was synthesized in 0.75% aqueous solution of CTS-g-PVP. The size of torispherical nano-silver particles are about 20 nm to 30 nm. The particles dispersed evenly in composites material with the amount of nano-silver in 1.51%wt. The composite material shows good biocompatibility and anti/bacteriostasis by using either alone or in the compound. Conclusions: With good anti/bacteriostasis effect and low irritation, Nano-Ag@CTS-g-PVP would have been used in the field of cosmetics.