基于近红外光谱和GC-MS法对油樟叶中挥发油的抗氧化研究和化学组成分析
Antioxidant Activity and Chemical Composition Analysis of Essential Oil from Cinnamomum longepaniculatum by NIRS and GC-MS
摘要: 本研究采用无溶剂微波辅助萃取法(SFME)和传统水蒸馏法(HD)获得油樟精油。通过单因素试验探究了无溶剂微波萃取法的最优条件。所得油樟精油萃取量为2.2 mL/100 g原料，水蒸气蒸馏法获得油樟精油的产量为1.6 mL/100 g。通过单因素分析法确定SFME的最佳萃取条件为微波功率700 W，微波处理时间25 min。用GC-MS分析SFME和HD萃取的油樟精油的化学成分，油樟挥发性成分由烯烃，醇类和少量的酯类化合物组成。SMFE法获得的精油中，相对含量最高的为桉叶油醇(相对含量为67.07%)；其次是α-松油醇(相对含量为11.33%)；桧烯含量也较高(相对含量为10.98%)。HD法获得的精油中，成分含量最高的也是桉叶油醇(相对含量为53.84%)，其次是α-松油醇(相对含量为14.89%)，桧烯含量也较高(相对含量为10.48%)。在DPPH、ABTS自由基清除实验中，50%浓度的精油样品对DPPH阴离子自由基的清除率为96.50%，5%浓度的精油样品对ABTS阳离子自由基的清除率为96.35%。可见油樟精油对两种自由基具有较高的清除率，且对ABTS阳离子自由基的清除能力高于对DPPH阴离子自由基的清除能力。采用近红外技术(NIRS)对样品进行红外光谱分析来探究油樟精油的抗氧化类型，为今后的对油樟挥发油的研究提供数据支持。
Abstract: In this work, essential oil of Cinnamomum longepaniculatum was obtained by solvent-free micro-wave assisted extraction (SFME). Compared with hydro-distillation (HD) extraction, SFME can get higher yield of essential oil. Through the single factor analysis, the optimum extraction parameters of SFME were extraction time 25 min, irradiation power 700 W. The extraction yield of essential oil was 2.2 mL/100 g. While the extraction yield of essential oil was 1.6 ml/100 g by HD. The chemical constituents of essential oil from Cinnamomum longepaniculatum extracted by SFME and HD were analyzed by GC-MS. The volatile components are composed of alkenes, alcohols and a small amount of esters. In the essential oil obtained by SFME, the content of eucalyptol was the highest (RA% = 67.07%), followed by α-terpineol (RA% = 11.33%), and then sabinene (RA% = 10.98%). In the essential oil obtained by HD, the content of eucalyptol was also the highest (RA% = 53.84%), and then α-terpineol (RA% = 14.89%), and sabinene (RA% = 10.48%) was higher. The DPPH and ABTS radical scavenging experiments were used to value the antioxidant activity of essential oil from Cinnamomum longepaniculatum. Moreover, NIRS provided more evidence to prove the antioxidant type of essential oil directly.
文章引用：王艺蓓, 孙莉寒, 武剑栋, 马春慧. 基于近红外光谱和GC-MS法对油樟叶中挥发油的抗氧化研究和化学组成分析[J]. 植物学研究, 2018, 7(3): 251-259. https://doi.org/10.12677/BR.2018.73032

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