TiO膜表面固定硒代胱氨酸及其内皮细胞相容性研究

Study on Selenocystine Immobilization on TiO Surface and It’s Endothelial Cell Compatibility

摘要: 内皮细胞相容性是心血管材料植入人体后能否持续发挥功能的重要评价指标，材料释放一氧化氮(NO)可能影响内皮细胞的生长、粘附、增殖等行为。本文采用聚多巴胺作为中间连接层，将硒代胱氨酸固定在钛氧薄膜表面，利用硒代胱氨酸催化内源性NO供体释放NO。XPS结果显示，在含硒样品上出现了明显的Se3d峰，NO释放实验证明，含硒样品均能够稳定催化内源性NO供体持续释放NO，且释放速率与健康内皮细胞NO释放速率一致。TiO样品表面具有较好的内皮相容性，在未添加NO供体时内皮细胞粘附数量多，优于固定硒代胱氨酸的样品。在添加了内源性供体条件下，固定硒代胱氨酸表面内皮细胞粘附数量显著增加，并且随着NO释放速度增加而进一步增强，内皮细胞的粘附数量均优于Ti-O样品，同时NO释放还促进了内皮细胞在样品表面的迁移。综合以上结果，表面固定硒代胱氨酸，通过催化内源性供体释放NO促进了内皮细胞的粘附及迁移，改善了材料表面的内皮细胞相容性。

Abstract: Endothelial cell compatibility is one of the important factors to determine the in vivo function of cardiovascular implant. Nitric oxide (NO) release from material may affect growth, adhesion and proliferation of endothelial cells. In this paper, polydopamine was used as linking layer, then se-lenocystine was immobilized on the surface of titanium oxide film via polydopamine. And NO was released from endogenous NO donor catalyzed by selenocysteine immobilized surfaces. Results of XPS showed that there were obvious Se3d peaks in Se-containing samples. Results showed that Se-containing samples could stably catalyze endogenous NO donors to release NO sustainably. It showed the release rate was consistent with that of healthy endothelial cells. TiO films had good endothelial cell compatibility and the number of adhered endothelial cell was more than that of samples immobilized with selenocystine without nitric oxide donor. However, when endogenous NO donor was added, adhesion number of endothelial cells on selenocystine immobilized surface increased significantly with NO release. The number of adhered endothelial cells was more than that of TiO samples. NO release also promoted migration of endothelial cells on the surfaces. In conclusion, immobilization of selenocystine on the surfaces promoted adhesion and migration of endothelial cells by catalyzing release of NO, and then improved endothelial cell compatibility.