microRNA-224在胆管癌患者中的表达及其临床病理学意义
Expression of microRNA-224 in Cholangiocarcinoma and Its Clinicopathological Significance
DOI: 10.12677/ACRPO.2018.72002, PDF,    科研立项经费支持
作者: 王小磊, 刘小方：青岛大学附属烟台毓璜顶医院，山东 烟台
关键词: 胆管癌；miR-224；临床病理；Cholangiocarcinoma； miR-224； Clinicopathology
摘要: 目的：探讨miR-224在胆管癌组织和癌旁组织中的表达差异及其临床病理学意义。方法：利用RT-PCR技术检测30例胆管癌患者癌组织及癌旁组织中miR-224的表达，并运用统计学方法分析其与临床病理资料之前的关系。结果：胆管癌组织中miR-224-5p的表达水平明显高于癌旁组织中的表达水平(P < 0.0001)，其表达水平高低与肿瘤病理生理学行为相关，即有淋巴结转移的患者癌组织中的miR-224-5p的相对表达量明显高于无淋巴结转移的患者(P < 0.05)；III期与IV期胆管癌患者癌组织中miR-224-5p的相对表达量明显高于I期与II期患者(P < 0.05)。结论：micro-224在胆管癌中具有潜在的癌基因作用，可能调控肿瘤的发生发展，有望成为胆管癌诊治的分子标志物。
Abstract: Objective: To investigate the difference of miR-224 expression in cholangiocarcinoma and para-cancerous tissues and to explore its clinicopathological significance. Methods: The expression of miR-224 in 30 cases of cholangiocarcinoma and its paracancerous tissues was analyzed by RT-PCR. The relationship between miR-224 and clinicopathological data was analyzed by statistical analysis. Results: The expression level of miR-224-5p in cholangiocarcinoma was significantly higher than that in paracancerous tissue (P < 0.0001). The expression level of miR-224-5p in cholangiocarcinoma was correlated with the pathophysiological behavior of tumor. That is, the relative expression level of miR-224-5p in patients with lymph node metastasis was significantly higher than that in patients without lymph node metastasis (P < 0.05). The relative expression of miR-224-5p in stage III and IV cholangiocarcinoma was significantly higher than that in stage I and II (P < 0.05). Conclusion: Micro-224 is a potential tumor promoter in cholangiocarcinoma, which may regulate the occurrence and development of cholangiocarcinoma and be used as a molecular marker for the diagnosis and treatment of cholangiocarcinoma.
文章引用：王小磊, 刘小方. microRNA-224在胆管癌患者中的表达及其临床病理学意义[J]. 亚洲肿瘤科病例研究, 2018, 7(2): 9-15. https://doi.org/10.12677/ACRPO.2018.72002

参考文献

[1] Razumilava, N. and Gores, G.J. (2014) Cholangiocarcinoma. Lancet, 383, 2168-2179. [Google Scholar] [CrossRef] 
[2] Khan, S.A., Toldano, M.B. and Taylor-Robinson, S.D. (2008) Epidemiology, Risk Factors, and Pathogenesis of Cholangiocarcinoma. HPB (Oxford), 10, 77-82. [Google Scholar] [CrossRef] [PubMed]
[3] Sayed, D. and Abdellatif, M. (2011) Micrornas in Development and Diserse. Physiological Reviews, 91, 827-887. [Google Scholar] [CrossRef] [PubMed]
[4] Farazi, T.A., Hoell, J.I., Morozov, P., et al. (2013) MicroRNAs in Human Cancer. Advances in Experimental Medicine and Biology, 744, 1-20. [Google Scholar] [CrossRef] [PubMed]
[5] Chen, W., Fan, X.M., Mao, L., et al. (2015) MicroRNA-224: As a Potential Target for miR-Based Therapy of Cancer. Tumor Biology, 36, 6645-6652. [Google Scholar] [CrossRef] [PubMed]
[6] 汤朝晖, 田孝东, 魏妙艳, 等. 美国癌症联合委员会胆道恶性肿瘤分期系统(第8版)更新解读[J]. 中国实用外科杂志, 2017, 37(3): 248-254.
[7] Wang, J. and Sen, S. (2011) MicroRNA Functional Network in Pancreatic Cancer: From Boilogy to Biomarker of Disease. Journal of Biosciences, 36, 481-491. [Google Scholar] [CrossRef] [PubMed]
[8] Budhu, A., Ji, J. and Wang, X.W. (2010) The Clinical Potential of microRNAs. Journal of Hematology & Oncology, 3, 37. [Google Scholar] [CrossRef] [PubMed]
[9] Lan, S.H., Wu, S.Y., Zuchini, R., et al. (2014) Autophagysuppresses Tumorigenesis of Hepatitis B Virus-Associated Hepatocellular Carcinoma through Degradation of microRNA-224. Hepatology, 59, 505-517. [Google Scholar] [CrossRef] [PubMed]
[10] Liu, H., Li, P., Li, B., et al. (2014) RKIP Suppresses Gastriv Cancer Cell Proliferation and Invasion and Enhances Apoptosis Regulated by microRNA-224.Tumour Biology, 35, 100095-10103.
[11] Zhang, G.J., Zhou, H., Xiao, H.X., et al. (2013) Up-Regulation of miR-224 Promotes Cancercell Proliferation and Invasion and Predicts Relapse of Colorectal Cancer. Cancer Cell International, 13, 104. [Google Scholar] [CrossRef] [PubMed]
[12] 邹叶青, 刘川, 贺文凤, 等. 反义miＲ-224 对人胰腺癌细胞系Aspc-1和Bxpc-3侵袭、迁移的影响及机制研究[J].临床检验杂志, 2014, 32(3): 212-217.
[13] Ni, H., Wang, X., Liu, H., Tian, F. and Song, G. (2015) Low Expression of miRNA-224 Predicts Poor Clinical Outcome in Diffuse Large B-Cell Lymphoma Treated with RCHOP. Biomarkers, 20, 253-257. [Google Scholar] [CrossRef] 
[14] Wan, Y., Zeng, Z.C., Xi, M., et al. (2015) Dysregulated microRNA-224/Apelin Axis Associated with Aggressive Progression and Poor Prognosis in Patients with Prostate Cancer. Human Pathology, 46, 295-303. [Google Scholar] [CrossRef] [PubMed]
[15] Feng, X.B., Zhao, L., Gao, S.G., et al. (2016) Increased Fucosylation Has a Pivotal Role in Multidrug Resistance of Breast Cancer Cells through miR-224-3p Targeting FUT4. Gene, 578, 232-241. [Google Scholar] [CrossRef] [PubMed]
[16] Wang, F., Shan, S., Li, Y.M., et al. (2016) miR-224-3p Inhibits Autophagy in Cervical Cancer Cells by Targeting FIP200. Scientific Reports, 6, Article ID: 33229. [Google Scholar] [CrossRef] [PubMed]
[17] 徐志勇, 周建龙, 周娟, 等. MicroRNA-224-5p在非小细胞肺癌中的表达及其临床意义[J]. 实用医学杂志, 2016, 32(21): 3463-3467.