大白菜和甘蓝CBL基因的鉴定和比较分析
Identification and Comparation Analysis of Chinese Cabbage and Cabbage CBL Genes
DOI: 10.12677/HJAS.2018.84056, PDF,    国家科技经费支持
作者: 王永强, 杨桂兰, 杨宗辉：山东省农业科学院蔬菜花卉研究所，国家蔬菜改良中心山东分中心，山东省设施蔬菜生物学重点实验室，山东 济南；张 卓：辽宁省锦州市太和区果树技术推广站，辽宁 锦州；曹齐卫*：东省农业科学院蔬菜花卉研究所，国家蔬菜改良中心山东分中心，山东省设施蔬菜生物学重点实验室，山东 济南
关键词: 大白菜；甘蓝；钙调磷酸酶B亚基蛋白基因；结构；进化；顺式元件；Brassica rapa L.； Brassica olerecea L.； CBL Gene； Structure； Phylogeny； Cis-Element
摘要: 植物CBL基因在逆境应答及发育过程中具有重要功能。然而，有关重要蔬菜大白菜和甘蓝CBL的研究缺乏报道。笔者在大白菜和甘蓝CBL基因组解析的基础上，对它们进行了系统比较。结果发现，大白菜和甘蓝都有16个CBL基因，它们分别位于不同的染色体上，编码区序列大小相似，在序列组成、基因结构和内含子数目上相对保守，在顺式调控元件上存在一定差异。这说明大白菜和甘蓝的CBL家族成员存在功能上的分化。本研究为进一步解析大白菜和甘蓝CBL基因在逆境应答中的功能提供了有益启示。
Abstract: Plant CBL genes play important roles in stress response and development. However, knowledge about CBL genes in important vegetables Chinese cabbage and cabbage is scarce. In this text, systematic comparation was performed on the basis of genomic dissection about CBL genes of Chinese cabbage and cabbage. The results showed that there are 16 CBL genes in Chinese cabbage and cabbage genome respectively. All of them are located on different chromosomes. Their putative coding sequence size is similar, and their sequence composition, gene structure, and intron number are relatively conserved. But there is somewhat difference for their upstream ciselements, which suggests that the function of Chinese cabbage and cabbage CBL gene members are divergent. This in-vestigation will provide valuable information for further functional dissection of CBL genes of Chi-nese cabbage and cabbage in stress response.
文章引用：李利斌, 张卓, 王永强, 杨桂兰, 杨宗辉, 孟昭娟, 曹齐卫. 大白菜和甘蓝CBL基因的鉴定和比较分析[J]. 农业科学, 2018, 8(4): 349-359. https://doi.org/10.12677/HJAS.2018.84056

参考文献

[1] 沈金秋, 郑仲仲, 潘伟槐, 等. 植物CBL-CIPK信号系统的功能及其作用机理[J]. 植物生理学报, 2014, 50(4): 641-650.
[2] 董连红, 史素娟, Nuruzzaman Manik, S.M., 等. 植物CBL 基因家族的研究进展[J]. 核农学报, 2015, 29(5): 892-898.
[3] Sanyal, S.K., Rao, S., Mishra, L.K., et al. (2016) Plant Stress Responses Mediated by CBL-CIPK Phosphorylation Network. Enzymes, 40, 31-64. [Google Scholar] [CrossRef] [PubMed]
[4] 刘明毓, 纪复勤, 马强, 等. 甜瓜CBL 基因的鉴定和特征分析[J]. 山东农业科学, 2015, 47(10): 17-21.
[5] 刘淑梅, 王施慧, 刘明毓, 等. 番茄CBL家族基因的鉴定和遗传进化分析[J]. 分子植物育种, 2015, 13(10): 2268-2273.
[6] 曹齐卫, 刘明毓, 陈伟, 等. 黄瓜CBL 基因的鉴定和特征分析[J]. 核农学报, 2016, 30(11): 2127-2132.
[7] 刘思源, 黄海锋, 王旭东, 等. 辣椒全基因组中CBL、CIPK基因家族的鉴定及特性分析[J]. 分子植物育种, 2017, 15(8): 2977-2985.
[8] Quan, R., Lin, H., Mendoza, I., et al. (2007) SCABP8/CBL10, a Putative Calcium Sensor, Inter-acts with the Protein Kinase SOS2 to Protect Arabidopsis Shoots from Salt Stress. Plant Cell, 19, 1415-1431. [Google Scholar] [CrossRef] [PubMed]
[9] Lin, H., Yang, Y., Quan, R., et al. (2009) Phosphorylation of SOS3-LIKE CALCIUM BINDING PROTEIN8 by SOS2 Protein Kinase Stabilizes Their Protein Complex and Regulates Salt Tolerance in Ara-bidopsis. Plant Cell, 21, 1607-1619. [Google Scholar] [CrossRef] [PubMed]
[10] Ren, X., Qi, G., Feng, H., et al. (2013) Cal-cineurin B-Like Protein CBL10 Directly Interacts with AKT1 and Modulates K+ Homeostasis in Arabidopsis. Plant Journal, 74, 258-266. [Google Scholar] [CrossRef] [PubMed]
[11] Xu, J., Li, H.D., Chen, L.Q., et al. (2006) A Protein Kinase, Interacting with Two Calcineurin B-Like Proteins, Regulates K+ Transporter AKT1 in Arabidopsis. Cell, 125, 1347-1360. [Google Scholar] [CrossRef] [PubMed]
[12] Shi, S., Chen, W. and Sun, W. (2011) Comparative Proteomic Analysis of the Arabidopsis cbl1 Mutant in Response to Salt Stress. Proteomics, 11, 4712-4725. [Google Scholar] [CrossRef] [PubMed]
[13] Huang, C., Ding, S., Zhang, H., et al. (2011) CIPK7 Is Involved in Cold Response by Interacting with CBL1 in Arabidopsis thaliana. Plant Science, 181, 57-64. [Google Scholar] [CrossRef] [PubMed]
[14] Pandey, G.K., Cheong, Y.H., Kim, K.N., et al. (2004) The Calcium Sensor Calcineurin B-Like 9 Modulates Abscisic Acid Sensitivity and biosynthesis in Arabidopsis. Plant Cell, 16, 1912-1924. [Google Scholar] [CrossRef] [PubMed]
[15] Mähs, A., Steinhorst, L., Han, J.P., et al. (2013) The Calcineurin B-Like Ca2+ Sensors CBL1 and CBL9 Function in Pollen Germination and Pollen Tube Growth in Arabidopsis. Molecular Plant, 6, 1149-1162. [Google Scholar] [CrossRef] [PubMed]
[16] Eckert, C., Offenborn, J.N., Heinz, T., et al. (2014) The Vacuolar Calcium Sensors CBL2 and CBL3 Affect Seed Size and Embryonic Development in Arabidopsis thaliana. The Plant Journal, 78, 146-156. [Google Scholar] [CrossRef] [PubMed]
[17] Tang, R.J., Zhao, F.G., Garcia, V.J., et al. (2015) Tonoplast CBL-CIPK Calcium Signaling Network Regulates Magnesium Homeostasis in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America, 112, 3134-3139. [Google Scholar] [CrossRef] [PubMed]
[18] Cheong, Y.H., Sung, S.J., Kim, B.G., et al. (2010) Constitutive Overexpression of the Calcium Sensor CBL5 Confers Osmotic or Drought Stress Tolerance in Arabidopsis. Molecules and Cells, 29, 159-165. [Google Scholar] [CrossRef] [PubMed]
[19] Ma, Q., Tang, R., Zheng, X., et al. (2015) The Calcium Sensor CBL7 Modulates Plant Responses to Low Nitrate in Arabidopsis. Biochemical and Biophysical Research Communications, 468, 59-65. [Google Scholar] [CrossRef] [PubMed]
[20] Monihan, S.M., Magness, C.A., Yadegari, R., et al. (2016) Arabidopsis Cal-cineurin B-Like 10 Functions Independently of the SOS Pathway during Reproductive Development in Saline Conditions. Plant Physi-ology, 171, 369-379. [Google Scholar] [CrossRef] [PubMed]
[21] De La Torre, F.D., Gutierrezbeltran, E., Parejajaime, Y., et al. (2013) The Tomato Cal-cium Sensor Cbl10 and Its Interacting Protein Kinase Cipk6 Define a Signaling Pathway in Plant Immunity. The Plant Cell, 25, 2748-2764. [Google Scholar] [CrossRef] [PubMed]
[22] 刘雨萌, 兰金苹, 曹英豪, 等. 水稻类钙调磷酸酶亚基B蛋白质在叶片生长和白叶枯病抗性反应中的表达[J]. 植物学报, 2012, 47(5): 483-490.
[23] 武丽娜, 赵泓, 刘凡. 蔬菜抗渗透胁迫基因工程研究进展[J]. 分子植物育种, 2008, 6(6): 1167-1174.
[24] 李化银, 姜常松, 张一卉, 等. 大白菜、甘蓝和黑芥CBL1基因的比较分析[J]. 山东农业科学, 2014, 46(12): 1-3.
[25] 李利斌, 纪复勤, 刘立锋, 等. 大白菜CBL4基因的比较分析[J]. 山东农业科学, 2015, 47(6): 5-7, 11.

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