HPLC法同时测定洁尔阴洗液中绿原酸和蛇床子素的含量

Simultaneous Determination of Chlorogenic Acid and Osthole in Jieeryin Lotion by HPLC

摘要: 目的：建立HPLC法同时测定洁尔阴洗液中绿原酸和蛇床子素的含量，为完善该洗液质量评价方法提供依据。方法：采用ZORBAXSB-C18色谱柱(4.6 × 150 mm, 5 μm)，流动相为乙腈-0.2%甲酸水溶液(梯度洗脱)。流速为1.0 mL/min；柱温为30˚C；检测波长为324 nm。结果：绿原酸和蛇床子素进样量分别在0.010~0.149 μg (r = 0.9998)、0.029~0.572 μg (r = 0.9999)范围内线性关系良好；平均加样回收率(n = 6)分别为97.9%，100.5%；RSD分别为0.69%、0.96%。结论：本法适用于同时测定洁尔阴洗液中绿原酸和蛇床子素的含量。

Abstract: Objective: An HPLC method for the simultaneous determination of chlorogenic acid and osthole in jieeryin lotion was established, providing the basis for improving the jieeryin quality evaluation method. Methods: The HPLC method was performed on a ZORBAX SB-C18 (4.6 mm × 150 mm, 5 μm) column. The mobile phase was a solution of acetonitrile-0.2% formic acid solution (gradient elution) at a flow rate of 1.0 mL/min. The detection wavelength was 324 nm, and the column temperature was 30˚C. Results: The calibration curves were linear within the range of 0.010 - 0.149 μg (r = 0.9998) for chlorogenic acid, 0.029 - 0.572 μg (r = 0.9999) for osthole, respectively. The average recoveries (n = 6) of chlorogenic acid and osthole were 97.9% (RSD = 0.69%), 100.5% (RSD = 0.96%), respectively. Conclusion: The method can be used for simultaneous determination of chlorogenic acid and osthole in jieeryin Lotion.