具有一氧化氮可控催化释放的比伐卢定功能化涂层的生物相容性研究

The Study on Biocompatibility of BVLD-Functionalized Coating with Controlled NO Catalytic Release

摘要: 本研究采用等离子体聚合技术在316L SS表面沉积聚烯丙胺薄膜，在碱性条件下进一步固定单宁酸(TA)，为表面提供大量活性位点，进而通过迈克加成席夫碱反应共价接枝比伐卢定(BVLD)和硒代胱胺(SeCA)，构建促内皮、抗凝和抗增生的多功能仿生涂层。采用活化部分凝血活酶时间(APTT)评价其抗凝性能，并进行细胞增殖实验(CCK-8)评价涂层对内皮细胞和平滑肌细胞的影响。结果显示，构建的仿生涂层在促进内皮细胞生长的同时显著抑制了平滑肌的生长，此外，与未接枝BVLD的对照涂层相比，该仿生涂层还显著延长了凝血时间。

Abstract: In this study, tannic acid (TA) was immobile on the polyallylamine film (PPAam) applied on 316L stainless steel via plasma polymerization in alkaline aqueous solution to provide rich active sites followed by covalently grafting bivalirudin (BVLD) and selenocystamine (SeCA) through Michael addition reaction and Schiff base reaction, thereby constructing a multifunctional bionic coating which can exhibit anticoagulant and antiproliferative properties and additionally promote the regeneration of healthy endothelium. Anticoagulant properties were demonstrated by activated partial thromboplastin time (APTT). Additionally, cell proliferation assays were carried out to test influence of the coating on the endothelial cells (ECs) and smooth muscle cells (SMCs). The result showed that the constructed coating not only promoted the growth of endothelial cells, but simul-taneously inhibited the proliferation of smooth muscle cells. Furthermore, the coating dramatically prolonged clotting time compared with the control without BVLD grafted thereon.