AGCU Database Y30试剂盒技术指标评价及法医学应用

Evaluation and Forensic Applications of AGCU Database Y30 Kit

摘要: 测试AGCU Database Y30试剂盒的六色荧光标记复合PCR检测体系，评估其技术指标及在法医学的应用价值。采集300份无关男性样本，对AGCU Database Y30试剂盒性能(灵敏度、稳定性、准确性、种属特异性，对男女混合样本、常见普通检材样本及建库样本的扩增能力)进行测试。该试剂盒检测样本准确，且灵敏度较高，最小检出量为0.125 ng；同一来源不同载体样本具有同一性、种属特异性高、抗抑制性效果良好，女性DNA或其他男性DNA存在下(19:1)仍然分型准确，且在不同批次试剂间结果稳定，对于血液痕迹、唾液、骨骼等不同常见检材样本的检测结果具有一致性，不同PCR环境均可获得完整分型。AGCU Database Y30试剂盒可应用于法庭科学实际检案与建库实验。

Abstract: The 6-dye fluorescence-labelling multiplex PCR detection system of the AGCU Database Y30 kit was tested to evaluate its technical specifications and its application value in forensic pathology. 300 unrelated male samples were collected to validate the performance of the AGCU Database Y30 kit (sensitivity, stability, accuracy, species specificity, and the amplification capabilities of male and female mixed samples, common samples, and building database samples). The kit is accurate with high sensitivity in detecting samples, and the minimum detectable amount is 0.125 ng. Different carrier samples from the same source have the same identity, high species specificity, and good anti-inhibitory effect. It has high sensitivity in the presence of female DNA or other male DNA (19:1). Stable results can be acquired between different batches of reagents. It has consistency for common blood samples, saliva, bones, and other common samples, and it got complete typing in different PCR environments. The AGCU Database Y30 kit can be applied to forensic science inspections and database-building experiments.