人博卡病毒1型独特区VP1u抗体制备及应用
Preparation and Application of Antibodies against Antigen VP1-Unique of Human Bocavirus 1
DOI: 10.12677/OJNS.2018.63034, PDF,    国家自然科学基金支持
作者: 许 多, 刘文宽*, 游爱萍, 周志超, 邱淑燕, 李 炽, 辜淑君, 周 荣：呼吸疾病国家重点实验室，国家呼吸系统疾病临床医学研究中心，广州呼吸健康研究院，广州医科大学附属第一医院，广州医科大学，广东 广州
关键词: 人博卡病毒1型；VP1u；抗体；免疫荧光；Human Bocavirus 1； VP1u； Antibody； Immunofluorescence
摘要: 人博卡病毒1型(human bocavirus 1, HBoV1)是全球流行的重要呼吸道病毒，对其的研究尚处于初期阶段。本研究针对HBoV1的VP1独特区(VP1u)通过原核表达的方式进行蛋白表达，并通过纯化、免疫小鼠成功获得了蛋白的抗体血清，通过ELISA评价了抗体对相应蛋白及临床病毒阳性样品的效价，并在后者发现制备抗体在1:50稀释时A450对阴性对照有4倍升高。利用所制备的抗体成功用于HBoV1广州株(GU338055)反向遗传系统的VP1u的免疫荧光研究中。本研究成功制备了HBoV1抗原VP1u抗体并应用，为HBoV1后续深入研究提供了重要的工具，具有重要意义。
Abstract: Human bocavirus 1 (HBoV1) is an important respiratory virus in the world, and its research is still in its early stages. In this study, the antigen VP1-unique (VP1u) of HBoV1 was expressed and purified using prokaryotic expression. The antibody was successfully obtained from immunized mice, and then evaluated by ELISA against the corresponding protein and clinical HBoV1-positive samples. The A450 value had 4-fold higher in the positive antibody than the negative control against clinical HBoV1-positve samples at 1:50 dilution. The antibody was successfully used in the immunofluorescence study of VP1u of HBoV1 Guangzhou strain (GU338055) reverse genetic system. In this study, we successfully prepared and evaluated antibody against antigen VP1u of HBoV1. The study provided an important tool for the further study of HBoV1.
文章引用：许多, 刘文宽, 游爱萍, 周志超, 邱淑燕, 李炽, 辜淑君, 周荣. 人博卡病毒1型独特区VP1u抗体制备及应用[J]. 自然科学, 2018, 6(3): 237-245. https://doi.org/10.12677/OJNS.2018.63034

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